Filipin at low concentrations rapidly hemolyzes bovine erythrocytes. Of the compounds tested, which were known a constituents of cell membranes, only cholesterol and phosphatidyl-choline prevented hemolysis. A specific complex was formed only with cholesterol. Filipin treatment caused a release of cholesterol from erythrocytes. Since all cell sterol is located in the cell membrane, the hemolyti...

background mesenchymal stem cells (mscs) are ideal candidates for treatment of diseases. amniotic membranes are an inexpensive source of mscs (am-msc) without any donor site morbidity in cell therapy. adipose tissue derived stem cells (ascs) are also suitable cells for cell therapy. there is discrepancy in cd271 expression among mscs from different sources. in this study, the characteristics of...

The lateral membrane plays an important role in the mechanical stability of epithelial cell sheet in steady state. In addition, the lateral membrane is continuously remodeled during dynamic processes such as cell extrusion, cytokinesis, and intercellular cell movement. In wound healing, the lateral membrane must be built from flat and spread cells that had crawled into the area of the wound. Th...

introduction: electroporation is a practical technique used to transport the molecules across the cell membrane. the utilization of fluorescent molecules is the method widely used to evaluate the electropermeability of cell membrane as a result of pulse application. it is also possible to use mathematical methods to predict the changes caused in cell electropermeability as a result of the chang...

background and objective: lactic acid is widely used in the food, chemical and pharmaceutical industries. the major problems associated with lactic acid production are substrate and end-product inhibition, and by-product formation. membrane technology represents one of the most effective processes for lactic acid production. the aim of this work is to increase cell density and lactic acid produ...

PURPOSE Following a previous study that demonstrated a correlation between rhodopsin stability and the severity of retinitis pigmentosa (RP), we investigated whether predictions of severity can be improved with a regional analysis of this correlation. The association between changes to the stability of the protein and the relative amount of rhodopsin reaching the plasma membrane was assessed. ...

Oxidative injury to hemoglobin (Hb) leads to formation of methemoglobin (MetHb), reversible hemichromes (rHCRs), and irreversible hemichromes (iHCRs). iHCRs precipitate and form Heinz bodies that attach to the red cell membrane causing injury that leads to hemolysis. The molecular mechanisms of this membrane damage have not been fully elucidated. We have studied the effect of Hb oxidation produ...

Background: Bile acids (BAs) affect cellular membranes Results: BAs stabilize domains in plasma membranes, leading to reorganization of membrane proteins and signaling perturbations Conclusion: BAs affect cell function by modulating the stability of plasma membrane nanodomains Significance: These results suggest mechanisms for regulation of functional membrane domains and non-receptor mediated ...

modeling the heat and mass transport in micro channel is being used extensively in researches and industrial applications. the aim is optimizing fuel cell designs before building a prototype for engineering application. in this study, numerical, three-dimensional, single phase computational fluid dynamics model of a proton exchange membrane fuel cell with both the gas distribution flow channels...

proteases play a key role in protein digestion in ticks and other haematophagous insects. our understanding of blood meal digestion in digestive system of ticks can be very useful for better understanding of basic rules for control of ticks. cells of the midgut endocytose blood components. blood proteins uptake by midgut cells, suggesting the presence of proteases in the midgut cells. in this s...