Transport through the ionomer as both a membrane and in catalyst layer is critical for efficient fuel-cell operation. To gain this understanding, we explore behavior of gas, water, ion transport membranes thin films using novel microelectrode interdigitated electrodes. In work, several common (including PFSA AEM chemistries) are evaluated custom cell to determine mass parameters hydrogen oxygen...

The sorting of apical and basolateral proteins into vesicular carriers takes place in the trans-Golgi network (TGN) in MDCK cells. We have previously analyzed the protein composition of immunoisolated apical and basolateral transport vesicles and have now identified a component that is highly enriched in apical vesicles. Isolation of the encoding cDNA revealed that this protein, annexin XIIIb, ...

Microelectrode and tracer techniques were used to test for possible amino acid-H(+) co-transport in coleoptiles of Avena sativa L. cv. "Garry." The amino acid analogue alpha-aminoisobutyric acid (AIB) caused transient depolarization of the membrane potential. The absolute magnitude of the maximum depolarization was affected by the same factors that affected AIB transport. Both increased with hi...

The Ca(++) transport mechanism in the red cell membrane was studied in resealed ghost cells. It was found that the red cell membrane can transport Ca(++) from inside the cell into the medium against great concentration gradient ratios. Tracing the movement of (45)Ca infused inside red cells indicated that over 95% of all Ca(++) in the cells was transported into media in 20 min incubation under ...

PURPOSE To investigate effects of vascular endothelial growth factor (VEGF) on glucose transport and GLUT1 glucose transporter expression in primary bovine retinal endothelial cell (BREC) cultures. METHODS Glucose transport in control and VEGF-treated BREC cultures was determined by measurement of [14C]-3-O-methylglucose (3MG) uptake. GLUT1 protein and mRNA was determined by Western and North...

Intracellular transport of membrane organelles occurs along microtubules (MTs) and actin filaments (AFs). Although transport along each type of the cytoskeletal tracks is well characterized, the switching between the two types of transport is poorly understood because it cannot be observed directly in living cells. To gain insight into the regulation of the switching of membrane organelles betw...

Abstract Adhesion of T cells to antigen presenting is mediated by the TCR–MHCp and LFA1–ICAM1 protein complexes. These intercellular complexes segregate form characteristic special patterns in cell contact zone. Previous studies have attempted explain mechanisms formation these patterns. While emphasis has been put on membrane elasticity active cytoskeletal transport, it remains unclear whether...

comIn the present study, CO2 removal from natural gas stream has been studied using a flat sheet membrane contactor. A three dimensional mathematical model is developed to describe the process. The model considers the transport of a gas mixture containing carbon dioxide and methane through a flat sheet membrane contactor module. The model is based on the non-wetted mode of operation, in which t...

Dynamic and steady-state water flux, current density, and resistance across a Nafion 115 membrane-electrode-assembly (MEA) were measured as functions of temperature, water activity, and applied potential. After step changes in applied potential, the current, MEA resistance, and water flux evolved to new values over 3000-5000 s, indicating a slow redistribution of water in the membrane. Steady-s...