– Numerical simulation of hydrocyclones aiming at investigating the separation of microorganisms and mammalian cells was performed using Computational Fluid Dynamics (CFD). The turbulence model used in the 2daxisymmetric calculations was the Reynolds Stress Model (RSM), in order to take into account the high swirl effects that occur in this type of equipment, which induce anisotropic turbulence...

Dielectrophoretic field-flow-fractionation (DEP-FFF) was applied to several clinically relevant cell separation problems, including the purging of human breast cancer cells from normal T-lymphocytes and from CD34+ hematopoietic stem cells, the separation of the major leukocyte subpopulations, and the enrichment of leukocytes from blood. Cell separations were achieved in a thin chamber equipped ...

This review describes recent work in cell separation using micro- and nanoscale technologies. These devices offer several advantages over conventional, macroscale separation systems in terms of sample volumes, low cost, portability, and potential for integration with other analytical techniques. More importantly, and in the context of modern medicine, these technologies provide tools for point-...

Microfluidic continuous cell separation based on hydrodynamic interaction in a microfluidic channel has attracted attention because of its robustness, high throughput and cell viability. This paper systematically gives an overview on recent advances in hydrodynamic particle and cell separation in microfluidic devices. It presents the basic ideas and fluid mechanics for the hydrodynamic interact...

The cell is the basic functional unit within a tissue or an organ. Methods that can be used to probe the cell, so as to understand, or even manipulate its interrelated processes, pathways, and/or overall functioning, are of great scientific and commercial value. Research efforts in molecular biology, biochemistry, and biotechnology over the last two decades have created high demand for efficien...

Adhesion between plant cells is a fundamental feature of plant growth and development, and an essential part of the strategy by which growing plants achieve mechanical strength. Turgor pressure provides non-woody plant tissues with mechanical rigidity and the driving force for growth, but at the same time it generates large forces tending to separate cells. These are resisted by reinforcing zon...

This work presents a specifically designed spiral type inertial micro-fluidic channel for separating non-motile sperm cell from cellular debris. Demonstration with a mixture of non-motile sperm and red blood cells showed that 82% of sperm could be focused into a separate outlet from the red blood cells.

We propose a new hydrophoretic method for continuous blood cell separation using a microfluidic device composed of slanted obstacles and filtration obstacles. The slanted obstacles have a larger height and gap than the particles in order to focus them to a sidewall by hydrophoresis. In the successive structure, the height and gap of the filtration obstacles with a filtration pore are set betwee...

The ability to rapidly and accurately sort multiple types of biological targets-such as molecules, viruses, bacteria or mammalian cells-from complex sample mixtures is an essential component for a wide range of diagnostic and therapeutic strategies. However, most current selection methods for cell separation are either limited with regard to throughput, as is the case for Fluorescence Assisted ...

Systems biology along with what is now classified as cytomics provides an excellent opportunity for cytometry to become integrated into studies where identification of functional proteins in complex cellular mixtures is desired. The combination of cell sorting with rapid protein-profiling platforms offers an automated and rapid technique for greater clarity, accuracy, and efficiency in identifi...