Characterisation and evaluation of soil bacteria were conducted in order to select the most potent strains that participate degradation cellulose unique agroecosystem climatic conditions. Cellulolytic activity was estimated using qualitative assays such as growth on selective media followed by screening with Congo red, Gram’s iodine solution, confirmation test red agar, determination enzyme pro...

The rumen typically operates as an energy-limited, nitrogen-excess system, but diets can be so low in degradable crude protein that microbial growth is limited by N. Ruminal bacteria respond differently to N sources and N-limitation. Cellulolytic ruminal bacteria need ammonia as an N source, have little capacity to utilize amino-N, are unable to ferment fiber when ammonia is depleted, and do no...

Branched glycerol dialkyl glycerol tetraethers (bGDGTs) are predominantly found in soils and peat bogs. In this study, we analyzed core (C)-bGDGTs after hydrolysis of polar fractions using liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry and analyzed intact P-bGDGTs using total lipid extract (TLE) without hydrolysis by liquid chromatography-electrospray ionizatio...

Abstract. Rinady MVP, Nuraini Y, Prayogo C, Arfarita N. 2023. The effect of land management and organic matter inputs on bacterial population soil nutrients across different types agroforestry system. Biodiversitas 24: 1333-1345. Different systems changes crop composition growth. This will affect that become the source food energy for microorganisms. organisms themself play an important role in...

Cellulose degrading enzymes have important functions in the biotechnology industry, including the production of biofuels from lignocellulosic biomass. Anaerobes including Clostridium species organize cellulases and other glycosyl hydrolases into large complexes known as cellulosomes. In contrast, aerobic actinobacteria utilize systems comprised of independently acting enzymes, often with carboh...

This paper reports a study of the effect of three nuclear and one thermal power plants on the microbial ecology of soils. Populations of bacteria, actinomycetes, fungi, cellulolytic microbes, phosphate-solubilizing microbes and nitrogen-fixing microbes in the soil in the vicinity of each plant were studied. Soils were acidic at three sites, and moisture contents of the power plant soils were lo...

Four sheep were fed an alfalfa hay diet. Rumen content samples were collected three hours after feeding in order to total microorganism population (TP), solid attached population (SAP) and solid attached firmly population (SAFP). Fibrolytic specific activities (xylanase, CMCase and beta-glycosidases) were estimated by the amount of reducing sugars or p-nitrophenol released from the appropriate ...

The bioconversion of cellulose and hemicellulose to soluble sugars is important for global stabilization and a sustainable human society. Here, hundreds of cellulolytic bacteria were screened and isolated from soil, compost, and animal waste slurry in Jeju Island, South Korea. Among the isolates, three strains, SL9-9, C5-16, and S52-2, showing higher potential for practical uses were purified o...

In this study, we designed and evaluated a microalgal pretreatment method using cellulolytic bacteria that naturally degrades microalgae in their native habitat. Bacterial strains were isolated from each of two mollusk species in a medium containing 1% carboxymethyl cellulose agar. We selected nine bacterial strains that had endoglucanase activity: five strains from Mytilus chilensis, a Chilean...