The use of chemostat culture facilitates the careful comparison of different yeast strains growing in well-defined conditions. Variations in physiology can be measured by examining gene expression, metabolite levels, protein content, and cell morphology. In this protocol, we show how a combination of sample types can be collected during harvest from a single 20-mL chemostat in a ministat array,...

The consumption of ammonia as N-source, orthophosphate as P-source, and glucose as Csource was investigated in batch and chemostat cultures of Pseudornonas putida NCMB 1960. Consumption of nutrients and growth in cell numbers were compared to a general 'Droop' type mathematical model previously suggested. Important characteristics of the growth patterns in batch cultures such as depletion of mo...

In this paper, a chemostat model for concerning impulsive state feedback control strategy is proposed and investigated. We obtained sufficient conditions of global asymptotical stability of the system without impulsive state feedback control. And also did we obtain the system with impulsive state feedback control may have order one periodic solution, as well as, the sufficient conditions for ex...

It was investigated whether Escherichia coli cultured in a glucose-limited chemostat is able to grow with a series of sugars whose utilization is normally repressed during batch growth with glucose. Cells growing at dilution rates of 0.2, 0.3 and 0.6h-1 were able to immediately utilize and grow with fructose, mannose, maltose and ribose. Galactose was transported instantaneously but growth star...