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Capillary electrophoresis (CE) is a special case of using an electrical field to separate the components of a mixture. Electrophoresis in a capillary is differentiated from other forms of electrophoresis in that it is carried out within the confines of a narrow tube. To understand the behavior of molecules under the influence of an electrical field inside a capillary it is essential to understa...

Abstract The objective of this work was to evaluate microfluidic chip electrophoresis, known as lab-on-a-chip technique, for the detection milk adulteration using cheese whey in comparison with SDS-PAGE. Raw, pasteurized, processed at an ultra-high temperature (UHT), and powdered samples received increasing concentrations (0, 1, 2.5, 5, 10, 20, 30, 50, 100% v/v), were subjected electrophoresis ...

CIEF and CZE are coupled with LIF detection to create an ultrasensitive 2-D separation method for proteins. In this method, two capillaries are joined through a buffer-filled interface. Separate power supplies control the potential at the injection end of the first capillary and at the interface; the detector is held at ground potential. Proteins are labeled with the fluorogenic reagent Chromeo...

BACKGROUND Microchip capillary electrophoresis (CE) is a promising method for chemical analysis of complex samples such as whole blood. We evaluated the method for point-of-care testing of lithium. METHODS Chemical separation was performed on standard glass microchip CE devices with a conductivity detector as described in previous work. Here we demonstrate a new sample-to-chip interface. Init...

Microsatellite instability (MSI) is caused by a failure of the DNA mismatch repair system and occurs frequently in various types of cancer. Since sporadic MSI are associated with approximately 10 to 15% of colorectal, gastric or endometrial carcinoma and impact clinical prognosis, MSI analysis is an important tool in clinical research and molecular diagnostics. Given that conventional technique...

The UV-curable adhesive NOA81 (Norland Optical Adhesive) is a promising liquid photopolymer for low-cost microfluidic chip production. Different fabrication methods for NOA81 are suggested [1,2]. However, optical characterization for fluorescent based detection systems is missing. We present a comparison of fluorescent emission spectra of various NOA types as well as the time evolution of the s...