Taurine (Tau) is an exceptionally abundant free amino acid in the cytosol of inflammatory cells and especially in neutrophils. Taurine protects cells from self-destruction during processes that generate oxidants. The major function of Tau in leukocytes is to trap chlorinated oxidants (HOCl). Taurine reacts with HOCl to produce the long-lived compound taurine chloramine (TauCl). Previously, we h...

This study was conducted to investigate Serratia liquefaciens infection in juvenile cultured rainbow trout (Oncorhynchus mykiss Walbaum) during June and July in 1996 about 20 days after flooding in three farms in Erzurum. Bacterial isolates were identified from each farm and tested to determine sensitivity against 18 chemotherapeutants. In addition to minimum bactericidal concentrations (MBC) o...

Er:YAG lasers have been demonstrated the usefulness for disinfection of anterior teeth with single root canal. However there has been few articles about the antibacterial effects in molars with root canal isthmuses (RCIs). 160 roots containing RCIs were selected, prepared up to file #40, and sterilized. They were divided evenly into two groups using randomization method, and inoculated with eit...

Previous studies in our laboratory have suggested that an oxidation reaction is responsible for the actions of free radicals to decrease synaptic potentials. Recently we observed that free radicals both decreased depolarization-induced vesicular release and enhanced basal, nonvesicular release of the excitatory amino acid, [3H]L-glutamate. In order to evaluate the contribution of oxidative reac...

Mouse epidermal growth factor (EGF) was radioiodinated by six different direct iodination methods. The 125I-labeled EGF preparations were distinguished by analyzing the binding of the radioligand to the EGF receptor (EGFR)-containing human placental membranes. The receptor-binding affinity of EGF labeled with Chloramine T was less than the affinity of unlabeled EGF, which precluded an accurate ...

We studied insulin binding to human erythrocytes using two different 125I-insulin-tracers. Erythrocytes of 8 normal subjects were examined using [mono-125II-(Tyr A 14)insulin as tracer. Three of these erythrocyte preparations were examined simultaneously using [125I]insulin, which was randomly iodinated by the chloramine-T-method. Data were analysed by a computerized non-linear least-squares pr...

INTRODUCTION All drinking water municipalities share the same goal of providing their communities with a reliable source of safe drinking water. To achieve this goal, most water systems must treat their water. The type of treatment used varies depending on the size, source, and water quality. Disinfection protects public water systems (PWSs) from potentially dangerous microbes. The most common ...

In the present study, the effects of hypochlorous acid (HOCl), monochloramine (NH(2)Cl), glutamine-chloramine (Glu-Cl) and taurine-chloramine (Tau-Cl) on the formation of 12-lipoxygenase (LOX) metabolite, 12-HETE, and cyclooxygenase (COX) metabolites, TXB(2), and 12-HHT, from exogenous arachidonic acid (AA) in rat platelets were examined. Rat platelets (4x10(8)/ml) were preincubated with drugs ...

In an inflammatory state where HOCl is generated, glycine readily reacts with HOCl to produce glycine chloramine, an anti-inflammatory oxidant. Colonic delivery of celecoxib elicits anticolitic effects in a trinitrobenzene sulfonic acid-induced rat colitis model. Glycine-bearing celecoxib derivatives were prepared and evaluated as a colon-specific mutual prodrug acting on nuclear factor-κB (NFκ...