The pesticide PCP was shown to inhibit the Hill reaction in broken chloroplasts (I50 = 15 jam) and to quench chlorophyll fluorescence. Both effects require preillumination. In contrast to the common “phenol-type” inhibitors, neither inhibition of Hill reaction nor chlorophyll fluorescence quench were affected by pretreatment of chloroplast with trypsin instead o f preillumination. An inhibition...

Reflectance spectra from 400 to 750 nm were measured in Lake Kinneret, when low phytoplankton stock prevailed, with chlorophyll concentrations from 3.1 to 7.3 pg/l. Algorithms for estimation of chlorophyll (CHL) concentration were devised using reflectances in the blue and green region of the spectrum and natural solar induced fluorescence. The fluorescence was calculated either from the reflec...

Fluorescence spectroscopy is applied to study the influence of plasmon excitations in spherical Au nanoparticles on the optical properties of chlorophyll-containing light-harvesting complexes. The separation between the two nanostructures is controlled via silica layer with varied thickness. We observe strong increase of the emission intensity for a 12nm-thick spacer and the increase is accompa...

Absorption and fluorescence experiments on pheophytin and chlorophyll containing lipid bilayer vesicles are reported. Pheophytin aggregates on the vesicles are established from an additional red shifted band (at 695 nm) in the absorption spectrum. These aggregates contain pheophytin in an arrangement with the molecular planes of the porphyrin rings being parallel and cover about 10% of the vesi...

response of tea plants to b deficiency was studied in hydroponic medium under environmentally controlled conditions. plants height, number of leaves and dry matter production of shoot and root were significantly decreased by b deficiency. concentration of chlorophyll, carotenoids, anthocyanins and flavonoids was not affected by b deficiency in the young leaf, while a significant reduction of ch...

The fluorescence decay characteristics of the isolated light-harvesting chlorophyll a/b protein have been studied using low-intensity subnanosecond-resolution time-correlated single-photon counting. In the monomeric state in detergent micelles, the chlorophyll a/b protein exhibits biexponential decay (~t = 1.2 ns, ~'2 = 3.3 ns) with the two components having very similar weights. The decay para...

Chlorophyll b was first detectable after 10 minutes of illumination of etiolated pea seedlings (Pisum sativum L. var Greenfeast) with continuous white light. The chlorophyll a/b ratio decreased from 300 at 10 minutes to 15 after 1 hour. There was little change in the chlorophyll a/b ratio between 1 and 2 hours, and it declined to 3 between 2 and 5 hours of illumination. In red light, the time c...

Changes in the functional organization of chlorophyll occur during light-dependent chlorophyll accumulation in yellow mutants of Chlamydomonas reinhardti£ These changes were studied using single-photon counting techniques to determine the chlorophyll fluorescence decay kinetics at various times after transfer of de-greened cultures to the light. Several different yellow mutants were analyzed: y...

The techniques utilized to extract chlorophyll from plant materials are destructive and based on methods that use organic solvents. This study proposes the non-destructive quantification of chlorophyll and nitrogen concentration using a portable chlorophyll meter (SPAD-502). Further, was possible to establish relationships between chlorophyll fluorescence and SPAD-502 values. This methodology c...