OBJECTIVE To obtain well characterized immortalized murine chondrocyte cell lines. The cell lines were obtained from mature articular chondrocytes, instead of embryonal cells which are used in most other studies. METHODS Pieces of articular cartilage were cut from murine patellae and femoral heads. Chondrocytes were isolated by digestion with collagenase. These cells were cultured in monolaye...

in this study, chondrocyte culture was established for the first time in iran, and calcium alginate was used for longer culture of chondrocyte in vitro. the study was programmed in order to be used for future human chondrocyte transplantation. the cartilage specimen obtained from 50 patients who underwent total knee and hip operations in isfahan university of medical sciences. cartilage specime...

INTRODUCTION Chondrocytes experience a hypertonic environment compared with plasma (280 mOsm) due to the high fixed negative charge density of cartilage. Standard isolation of chondrocytes removes their hypertonic matrix, exposing them to nonphysiological conditions. During in vitro expansion, chondrocytes quickly lose their specialized phenotype, making them inappropriate for cell-based regene...

Runx2 (runt-related transcription factor 2) is an important transcription factor for chondrocyte differentiation as well as for osteoblast differentiation. To investigate the function of Runx2 in chondrocytes, we isolated chondrocytes from the rib cartilage of Runx2-deficient (Runx2-/-) mice and examined the effect of Runx2 deficiency on chondrocyte function and behavior in culture for up to 12...

Cytokines, particular IL-1beta, are elevated in osteoarthritic cartilage, and are believed to mediate the progressive loss of cartilage matrix and of chondrocytes often associated with osteoarthritis (OA). IL-1 promotes a catabolic phenotype in chondrocytes, increasing the expression of collagen-degrading metalloproteinases and of aggrecanase, while decreasing proteoglycan synthesis and promoti...

The association between obesity and osteoarthritis (OA) in joints not subjected to mechanical overload, together with the relationship OA metabolic syndrome, suggests that there are systemic factors related disorders involved phenotype of OA. aim this work is study effects palmitate oleate on cellular metabolism an “ vitro ” model human chondrocytes. TC28a2 chondrocyte cell line was used analyz...

Chondrogenesis is a multistep process that is essential for endochondral bone formation. Previous results have indicated a role for -catenin and Wnt signaling in this pathway. Here we show the existence of physical and functional interactions between -catenin and Sox9, a transcription factor that is required in successive steps of chondrogenesis. In vivo, either overexpression of Sox9 or inacti...

We examined the effect of an extracellular matrix (ECM), produced by either bovine corneal endothelial (BCE) cells or mouse PF HR-9 teratocarcinoma cells, on the ability of rabbit costal chondrocytes to re-express their phenotype once confluent. Rabbit chondrocytes seeded at low densities and grown on plastic tissue culture dishes produced a heterogeneous cell population composed of both overtl...