Transfusion of donor blood containing predominantly younger red cells with prolonged survival in vivo could significantly reduce the iron overload of patients requiring chronic transfusion. Age-dependent separation of red cells can be obtained by buoyant density centrifugation on isotonic solutions of arabino-galactane. By this technique, rabbit red cells were separated on a single layer of ara...

The application of staining with the guanine-cytosine specific fluorochrome chromomycin A3 (CMA) of citrus chromosomes to genetic studies is reviewed. When CMA staining is performed, the existence of characteristic CMA banding patterns with high levels of diversity and heterozygosity in citrus chromosomes is demonstrated. Similar CMA banding patterns have been observed in related species. Thus,...

BACKGROUND Sperm cells extracted from testes (TESE) have poor chromatin quality and motility. Various substances are used in the laboratory to increase sperm motility and improve the ART outcomes; however, there are few research which considered improving both sperm motility and chromatin quality. OBJECTIVE The aim of this investigation was to evaluate the improvement of the testicular sperm ...

The relative three-dimensional co-location of RNA polymerase I (RPI) and DNA was studied using confocal laser scanning microscopy during interphase and all the steps of mitosis in human cancerous cells. For each step of the cell cycle, immunolabeled RPI molecules and DNA specifically stained with chromomycin A3 were simultaneously imaged at high resolution through numerous optical sections. The...

The aim of this study was to evaluate the effect of sperm DNA damage and protamine deficiency on fertilization and embryo development post-intracytoplasmic sperm injection (ICSI), and also to assess the effect of protamine deficiency on DNA damage. Semen samples were collected from 28 patients participating in the ICSI programme. Following sperm preparation and ICSI, the remaining processed sem...

Flow cytometry studies for cellular DNA analysis were performed in 115 cases of non-Hodgkin's lymphoma, 53 of which had not received any prior chemotherapy or radiotherapy. DNA content was measured in ethanol fixed cells stained with chromomycin A3. According to the criteria of the International Working Formulation there were 43 low grade, 58 intermediate grade, and eight high grade lymphomas; ...

BACKGROUND Varicocelectomy is considered as standard treatment for male infertility for clinical varicocele. The aim of this study is to address the effects of varicocelectomy on semen parameters, chromatin packaging, and pregnancy outcome. MATERIALS AND METHODS This retrospective study was carried out between June 2006 and February 2011 on 145 infertile men with grade II or III varicocele. M...

Considering that the final protection of the DNA against major assaults in terms of chromatin condensation is finalized in the epididymis, it is not known how sperm production of reactive oxygen species (ROS) and inflammatory processes can contribute to protamine deficiency that is predetermined in the testes. Therefore, this study aimed at investigating relationships between poor chromatin con...

BACKGROUND Etiology of more than half of Recurrent Spontaneous Abortion. The etiology of more than 50 percent of Recurrent Spontaneous Abortions (RSA) cases has been remained unexplained. It is supposed that RSA may have "paternal effect" due to supply 50% of embryonic genomic content by male gamete. OBJECTIVE The aim of present study was to evaluate the role of sperm apoptosis and protamine ...

This is the first report implementing combined density gradient centrifugation/Zeta (DGC/Zeta) sperm selection procedure for a couple with eleven previous intra cytoplasmic sperm injection/ in vitro fertilization (ICSI/IVF) failure cycles. Semen analysis was carried out according to World Health Organization criteria. Protamine deficiency, DNA fragmentation and morphology were assessed by chrom...