The flagellin type III secretion pathway of Bacillus halodurans BhFC01 (Deltahag) was modified by the inactivation of fliD. An in-frame flagellin gene fusion polypeptide construct was expressed, and the heterologous peptides were secreted as flagellin fusion monomers. The stability of the secreted monomers was significantly enhanced through gene-targeted inactivation of extracellular proteases.

Abstract On the morning of 6 May 2021, military police invaded favela Jacarezinho, one Rio de Janeiro's slums, and killed 28 people during a operation tellingly named Operation Exceptis. Photos dead bodies in alleys denouncements extrajudicial executions individuals who had already surrendered circulated widely on internet. Jacarezinho adds to troubling record killings that includes goes far be...

In this paper, we present a method for fully automatic facial expression recognition in facial image sequences using feature extracted from tracking of facial landmarks. The facial landmarks at the first frame of the image sequence under examination are initialized using elastic bunch graph matching (EBGM) algorithm and tracked in the consecutive video frame over time. At first, the most discri...

Automatic facial Action Unit (AU) detection from video is a long-standing problem in facial expression analysis. AU detection is typically posed as a classification problem between frames or segments of positive examples and negative ones, where existing work emphasizes the use of different features or classifiers. In this paper, we propose a method called Cascade of Tasks (CoT) that combines t...

The generic matrix-matrix multiplication (GEMM) is arguably the most popular computational kernel of the 20th century. Yet, surprisingly, no common methodology for evaluating GEMM performance has been established over the many decades of using GEMM for comparing architectures, compilers and ninja-class programmers. We introduce GEMMbench, a framework and methodology for evaluating performance o...

The lamellipodium is an important structure for cell migration containing branched actin nucleated via the Arp2/3 complex. The formation of branched actin is relatively well studied, but less is known about its disassembly and how this influences migration. GMF is implicated in both Arp2/3 debranching and inhibition of Arp2/3 activation. Modulation of GMFβ, a ubiquitous GMF isoform, by depletio...

Anaplastic lymphoma kinase (ALK) fusion is a common mechanism underlying pathogenesis of non-small cell lung carcinoma (NSCLC) where these rearrangements represent important diagnostic and therapeutic targets. In this study, we found a new ALK fusion gene, SEC31A-ALK, in lung carcinoma from a 53-year-old Korean man. The conjoined region in the fusion transcript was generated by the fusion of SE...

Recent studies have reported that the majority of prostate cancers express fusion genes in which the 5' region of the androgen-regulated TMPRSS2 gene is fused to an ETS family transcription factor, most commonly the ERG gene. We have characterized in detail the expression of TMPRSS2/ERG fusion mRNAs and correlated the isoforms expressed and expression levels with clinical outcome in cancers fro...

The mixed lineage leukemia (MLL) locus is involved in more than 60 different rearrangements with a remarkably diverse group of fusion partners in approximately 10% of human leukemias. MLL rearrangements include chromosomal translocations, gene internal duplications, chromosome 11q deletions or inversions and MLL gene insertions into other chromosomes, or vice versa. MLL fusion partners can be c...