objective(s):multi-epitopic protein vaccines and direction of vaccine delivery to dendritic cells (dcs) are promising approaches for enhancing immune responses against mutable pathogens. escherichia coli is current host for expression of recombinant proteins, and it is important to optimize expression condition. the aim of this study was the optimization of multi-epitopic hiv-1 tat/pol/gag/env ...

Plukenetia volubilis or commonly known as sacha inchi is reported to produce wide range of health-promoting bioactive metabolites. These metabolites functions supplements in eradicating various types diseases. Sacha has large edible seeds that are rich phenolic content, minerals and essential fatty acid, such omega 3 (ω-3), 6 (ω-6), 7 (ω-7), 9 (ω-9). In vitro cultures could serve alternative pr...

In this study, silver nanoparticles were synthesized  using the fungus, Aspergillus fumigatus. The effects of three independent variables including glucose content of culture media, initial pH and initial spore concentration on biosynthesis of silver nanoparticles were investigated. These variables affect cell morphology, cell mass, size and morphology of silver nanoparticles and degree of silv...

Pyricularia oryzae (Tel. Magnaporthegrisea) is currently used as a fungal model for plantmicrobeinteraction studies as well as an indicative model for anticancer drug discovery. Thepresent study introduces the optimal condition in which P. oryzae grows and sporulates beston common culture media. We have considered three fungal culture media, i.e. PDA, PCA andWA, based on which P. oryzae sporula...

pyricularia oryzae (tel. magnaporthegrisea) is currently used as a fungal model for plantmicrobeinteraction studies as well as an indicative model for anticancer drug discovery. thepresent study introduces the optimal condition in which p. oryzae grows and sporulates beston common culture media. we have considered three fungal culture media, i.e. pda, pca andwa, based on which p. oryzae sporula...

The aim of this paper is to present and critically discuss the long journey of the enterprise of culture from the modernist condition of “high culture” (or “elite culture”) to the contemporary postmodernist (?) condition of “popular culture” (or “mass culture”). It will particularly seek to illustrate and assess the nature, role and implications of the various “modes of reading and interpreting...

An in vitro organotypic culture model is needed to understand the complexities of carcinoma tissue consisting of carcinoma cells, stromal cells, and extracellular matrices. We developed a new in vitro model of carcinoma tissue using a rotary cell culture system with four disposable vessels (RCCS-4D) that provides a simulated microgravity condition. Solid collagen gels containing human pancreati...

The effect of site of supplementation of a mixture of two crude preparations (Enzyme C and Enzyme X) of exogenous polysaccharide-degrading enzymes (EPDE) was studied in vivo using four ruminally and duodenally cannulated heifers (Exp. 1). The treatments were as follows: control (no EPDE), EPDE supplied through the diet (EF, 47.0 g/d), and EPDE infused continuously into the abomasum (EA, 41.6 g/...