The organometallic complex coenzyme B12 (adenosyl cobalamin, AdoCbl) is not only an essential coenzyme in many biochemical reactions of most if not all living organisms but has lately been shown to play a crucial role in the regulation of B12 related genes. As a consequence, coenzyme B12 has been a target of intense research. However, the investigations of AdoCbl have often been hampered due to...

Objective: Vitamin B12 deficiency can lead to serious health problems both in children and adult. Intramuscular injection therapy is applied as the gold standard treatment of vitamin deficiency. There are also oral, nasal, sublingual spray methods. In this study, we aimed show efficacy children. Materials Methods: Forty-five pediatric patients (25 boys, 20 girls), aged 9-36 months with serum co...

This review apprises the reader of recent advances in cobalamin absorption and transport since 1970. This field has been of interest to gastroenterologists, hematologists, and biochemists as well as nutritionists. Clinical conditions leading to cobalamin deficiency present especially to the first two of these specialties. Many recent advances involve biochemical definition of the specific trans...

We describe a radioassay for cobalamin (vitamin B12) in human serum or plasma that requires no boiling or other pretreatment of the sample. Normal chicken serum covalently coupled to magnetizable particles is used as the binding agent. The assay is performed at pH 12.9, at which pH all cobalamin in human serum is released from its binding proteins, whereas the binding agent maintains a high aff...

Recent evidence (Kolhouse et aL, N. EngI. J. Med. 299: 785-792, 1978) demonstrates that commercial cobalamin (Vitamin B12) radioassay kits contain nonspecific R-protein bindingagents thatcan give falsely normal results in patients who are actually cobalamin deficient. We tested three kits:with“purified” intrinsic factor as the binder, with intrinsic factor and the nonspecific R-protein sites bl...

It has been established from studies with Escherichia coli mutants (1,2) and animal liver (3,4) that 1\Is-methyltetrahydrofolic acid (5-CH3-folate-H4) transfers its methyl group to homocysteine to form methionine. The functions of the complex requirements for this reaction (reduced flavin (1,5), S-adenosylmethionine (AMe)l(4), and a cobalamin compound (6-8)) have not yet been fully elucidated. ...

BACKGROUND This study aimed to determine red blood cell (RBC) and serum folate and vitamin B12 levels as well as their intake in schizophrenic patients. METHODS The folate and cobalamin status of 60 schizophrenic patients (15-55 years) was compared to 60 matched healthy controls using Radio Isotope Dilution Assay (RIDA). RESULTS Serum and RBC folate in schizophrenic patients was significant...

BACKGROUND: This study aimed to determine red blood cell (RBC) and serum folate and vitamin B12 levels as well as their intake in schizophrenic patients. METHODS: The folate and cobalamin status of 60 schizophrenic patients (15-55 years) was compared to 60 matched healthy controls using Radio Isotope Dilution Assay (RIDA). RESULTS: Serum and RBC folate in schizophrenic patients was significantl...

BACKGROUND It is unclear whether homocysteine itself is causal in the pathogenesis of cardiovascular disease. Alternatively or additionally, the association between homocysteine and cardiovascular disease may be because of its metabolic precursor, S-adenosylhomocysteine, or of the ratio of S-adenosylmethionine to S-adenosylhomocysteine. Therefore, it is relevant to know how these moieties are i...