This article presents an experimental study on the fatigue behaviour of cracks emanating from cold-expanded holes utilizing thermoelastic stress analysis (TSA) and synchrotron X-ray diffraction (SXRD) techniques with the aim of resolving the long-standing ambiguity in the literature regarding potential relaxation, or modification, of beneficial compressive residual stresses as a result of fatig...

The present study was undertaken to identify the nature of the interactive effects of chlorpyrifos (CPF) and cold stress (15 degrees and 20 degrees C) on the activities of acetyl cholinesterase (AChE), choline acetyl transferase (ChAT), Na+, K(+)-ATPase and malondialdehyde (MDA) level in the cerebral cortex of 1 week, 3 weeks and 3 months of age. The results indicated an interaction of CPF with...

To study low-temperature signaling in plants, we previously screened for cold stress response mutants using bioluminescent Arabidopsis plants that express the firefly luciferase reporter gene driven by the stress-responsive RD29A promoter. Here, we report on the characterization and cloning of one mutant, frostbite1 (fro1), which shows reduced luminescence induction by cold. fro1 plants display...

Complex proteomic and physiological approaches for studying cold and heat stress responses in plant mitochondria are still limited. Variations in the mitochondrial proteome of cauliflower (Brassica oleracea var. botrytis) curds after cold and heat and after stress recovery were assayed by two-dimensional polyacrylamide gel electrophoresis (2D PAGE) in relation to mRNA abundance and respiratory ...

Full-length cDNAs are essential for functional analysis of plant genes. Using the biotinylated CAP trapper method, we constructed full-length Arabidopsis cDNA libraries from plants in different conditions, such as drought-treated, cold-treated, or unstressed plants, and at various developmental stages from germination to mature seed. We prepared a cDNA microarray using approximately 1300 full-l...

To the Editor: In the rat (adult male Sprague-Dawley) chronic forced swim stress (Dilsaver et al. 1986) and chronic inescapable footshock (Dilsaver and Alessi 1987) induce marked supersensitivity to the hypothermic effects of the muscarinic cholinergic agonist oxotremorine. These observations are consistent with a report that immobilization of 10 min duration activates the septohippocampal musc...

Deschampsia antarctica is an Antarctic hairgrass that grows on the west coast of the Antarctic peninsula. In this report, we have identified and characterized a transcription factor, D. antarctica C-repeat binding factor 7 (DaCBF7), that is a member of the monocot group V CBF homologs. The protein contains a single AP2 domain, a putative nuclear localization signal, and the typical CBF signatur...