Due to the slow growth rate and pathogenicity of mycobacteria, enumeration by traditional reference methods like colony counting is notoriously time-consuming, inconvenient and biohazardous. Thus, novel methods that rapidly and reliably quantify mycobacteria are warranted in experimental models to facilitate basic research, development of vaccines and anti-mycobacterial drugs. In this study we ...

OBJECTIVE To compare the number of bacterial counts in tap water at first burst and running tap water. MATERIAL AND METHOD The present study was performed in thirty-two first burst water samples and twenty-nine running tap water samples after two minutes, collected from an operation room at one standard hospital and analyzed for the mean, median, and 95% achieved confidence interval of CFU/ml...

BACKGROUND Cyclophosphamide plus thalidomide as induction for multiple myeloma patients eligible for autologous stem cell transplantation may be a limiting factor for cell mobilization. The minimum acceptable mobilized peripheral blood stem cell count to prevent deleterious effects during transplantation is 2.0×106 CD34+ cells/kg. Combining other treatments to granulocyte-colony stimulating fac...

OBJECTIVE To evaluate the effectiveness and safety of the mobilization of peripheral blood hematopoietic stem cells by combining docetaxel with granulocyte colony-stimulating factor (G-CSF) in breast cancer patients. METHODS A total of 57 breast cancer patients were treated with docetaxel 120 mg/m(2). When the white blood cell (WBC) count decreased to 1.0×10(9)/L, patients were given G-CSF 5 ...

Beverages in aluminium cans are widely available, and for convenience, drinks often consumed directly from the orifice of can. Cans exposed to various environments during production, storage, transportation handling at retail shops which lid may be contaminated with microorganisms. This study was aimed isolating identifying bacteria surfaces canned offered sale on main campus Plateau State Poly...

BACKGROUND Toothbrushes may play a significant role in plaque control. Toothbrushes should be correctly stored, disinfected and changed at regular intervals. OBJECTIVE The purpose of this study was to evaluate the efficacy of 0.2% chlorhexidine (CHX) gluconate solution and ultra violet (UV) toothbrush-sanitizer for toothbrush disinfection. MATERIALS AND METHODS Fresh tooth brushes were dist...

BACKGROUND This study investigated the in vitro and in vivo antibacterial effects of three mouthwashes on supragingival plaque microbiota. The three mouthwashes under study were 0.2% chlorhexidine (CHX), Listerine®, and Persica (PM). Water was used as negative control. METHODS Supragingival plaque samples were collected from 32 patients with gingivitis in the Dental School of Shahid Beheshti ...

Root canal disinfection is one of the main factors governing success of endodontic therapy. Antimicrobial photodynamic therapy (aPDT) is presented as a promising antimicrobial therapy that can eliminate microbiota present in infected root canal systems. In this study, a series of experiments investigated the effects of aPDT on cell viability and biofilm degradation ability of endopathogenic mic...

Graphene oxide (GO) was synthesized by oxidation of graphite powder using a time-saving modification of Hummers’ method and its antibacterial activity was investigated. Different techniques were applied to characterize the synthesized GO. X-ray Diffraction (XRD), Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM) were used to investigate the crystallinity, morphology, topograp...

purpose: to determine bacterial flora of the conjunctiva and lacrimal sac in adult patients with naso-lacrimal duct obstruction (nldo) in comparison with normal flora. methods: this study included 40 consecutive adult patients with nldo and 40 age- and sex matched healthy controls. specimens for bacterial culture were obtained from the involved and normal conjunc-tiva preoperatively and from th...