A total of 37 street vended food samples were examined for bacterial and the colony forming units counts ranged from 4.5 × 10⁵ to 1.12 × 10⁶. The isolates were identified as Escherichia coli (37.5%), Pseudomonas aeruginosa (3.57%), Staphylococcus aureus (14.20%), Salmonella sp. (5.36%), Klebsiella sp. (10.71%), Shigella sp. (19.64%) and Enterobacter sp. (8.93%) respectively. All the 56 isolates...

Thirty clinical isolates of Staphylococcus aureus were tested for tolerance to oxacillin, cephalothin, and gentamicin by using killing curves and broth dilution susceptibility tests. Tolerance was defined as the presence of>/=100 colony-forming units per ml remaining after 24 h of incubation of the killing curve suspensions. Nineteen of 30 isolates were tolerant to oxacillin and cephalothin, bu...

PURPOSE The purpose of this study was conducted to determine if bristle wear impacts the adherence of Streptococcus mutans on toothbrushes and to evaluate whether it affects the extent of adherence at 0, 8, and 24 hours after air-drying. METHODS Sixty toothbrushes--composed of 20 each from 3 different groups and defined by brand, brush trim, and head shape--were used in this study. Bristle we...

The quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (OD600) measurements were used to evaluate Mycobacterium tuberculosis growth. Turbidity and OD600 measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. We determined the cfu equivalents to McFarland and OD600 units.

We developed a simple, less laborious method to cultivate and isolate obligate anaerobic microorganisms using a six-well plate together with the AnaeroPack System, designated as the six-well plate method. The cultivation efficiency of this method, based on colony-forming units, colony formation time, and colony size, was evaluated with four authentic obligate anaerobes (two methanogenic archaea...

Sterilized soils are commonly used for the study of xenobiotic sorption and as an abiotic control in biodegradation experiments. They are also used for the chemical study of nitrogen and carbon cycle processes and the elimination of the biological factors. In this research the effects of different soil sterilization methods such as autoclaving, fumigating and exposure to ultraviolet and microwa...

Consumption of produce contaminated with Escherichia coli O157:H7 has resulted in cases of foodborne illness. We determined the efficacy of a mixture of three E. coli O157:H7-specific bacteriophages (ECP-100) in reducing the number of viable E. coli O157:H7 on contaminated fresh-cut iceberg lettuce and cantaloupe. E. coli O157:H7 was spot inoculated on lettuce pieces (9 cm2) with a population o...

objective(s): ex vivo expansion of hematopoitic stem cells is an alternative way to increase umbilical cord blood (ucb)-cd34+ cells for bone marrow transplantation. for this purpose demineralized bone matrix (dbm) and mineralized bone allograft (mba) as two scaffolds based on bone matrix and stem cell niche, were simultaneously used to enhance the effect of human mesenchymal progenitor cells (m...

Hemopoiesis is disturbed in bone marrow-involving cancers like leukemia and neuroblastoma. Shedding of gangliosides by tumor cells may contribute to this tumor-induced bone marrow suppression. We studied in vitro the inhibitory effects of murine neuroblastoma cells (Neuro-2a and C1300) and their gangliosides on hemopoiesis using normal murine hemopoietic progenitor colony-forming assays. Transw...

The susceptibility of suckling mice to oral infection with several different Vibrio cholerae was determined. Mice up to 10 days of age were uniformly susceptible to oral infection with 10(8) colony-forming units of virulent organisms. Age-dependent resistance occurred thereafter to a maximum at about 15 days of age. The efficacy of selected vaccines was compared by oral challenge of 8-day-old, ...