The objective of the work was to evaluate two ELISA’s for detection anti-Map antibodies. 460 serum and fecal samples bovines (n=267), ovines (n=140), goats (n=53) were evaluated. Serums used detecting antibodies using different ELISAs, one in-house ELISA made with low-molecular weight proteins (<100 kD) from antigen ultra-filtrate Map strain 3065, a commercial paratuberculosis diagnost...

In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (D...

The objective was to compare deoxynivalenol (DON) concentrations in feed ingredients and commercial swine diets measured by enzyme-linked immunosorbent assay (ELISA) high-performance liquid chromatography (HPLC). Seventy ingredient samples consisted of corn, corn dried distillers grains with solubles, gluten feed, meal, palm kernel expellers, rice bran, soy hulls, soybean wheat. Commercial diet...

The aim of this study in Iraq was to determine the sensitivity and specificity of a commercial ELISA test for detection of Giardia lamblia antigen in stool. Of 84 stool samples from children in Duhok governorate, 42 were positive and 42 negative for G. lamblia or other parasites by direct and indirect microscopic examination. The sensitivity of the ELISA test for detection of G. lamblia versus ...

For detection of infectious laryngotracheitis virus (ILTV) antibody, glycoprotein B-, C-, and D-based enzyme-linked immunosorbent assays (B-, C-, and D-ELISAs, respectively) were developed. The B- and D-ELISAs showed enhanced detection of anti-ILTV antibodies in infected chickens compared to that of the commercial ELISA. Furthermore, the D-ELISA was efficient in detecting seroconversion with ve...

We evaluated two new commercial dengue diagnostic tests, the MRL Diagnostics Dengue Fever Virus IgM Capture ELISA and the PanBio Rapid Immunochromatographic Test, on serum samples collected during a dengue epidemic in Jamaica. The MRL ELISA method correctly identified 98% (78 of 80) of the samples as dengue positive, while the PanBio test identified 100% (80 of 80). Both tests were 100% (20 sam...

background: the assays currently available for the detection of specific anti- toxoplasma antibodies may vary in their abilities to detect serum immunoglobulins, due to the lack of a purified standardized antigen. the aim of this study was evaluation the recombinant toxoplasma gondii sag1 antigen for the serodiagnosis of acute and chronic toxoplasmosis. methods : this study describes an elisa u...

Infectious bronchitis (IB) is a very contagious disease caused by a coronavirus (IBV). In chickens, thevirus affects the respiratory, reproductive, and urinary systems. This study was carried out to determine theseroprevalence of anti-IBV antibodies in domestic village chickens. Serum samples of 300 domestic villagechickens from Esfahan (centeral Iran) were collected and examined for the presen...

The modified agglutination test (MAT) and a commercially available enzyme-linked immunosorbent assay (ELISA) were compared for detection of antibodies to Toxoplasma gondii in naturally-infected market-aged pigs. Infected pigs were obtained from commercial slaughter facilities and from farms where infection had previously been detected. Infection was confirmed by bioassay in cats. For 70 bioassa...