Geometric measures of localized calcium release (LCR) events have been used to understand their biophysical basis. We found power law scaling between three such metrics-maximum amplitude (MA), mass above half-maximum amplitude (MHM), and area at half-maximum amplitude (AHM). In an effort to understand this scaling a minimal analytic model was employed to simulate LCR events recorded by confocal...

Microscopical images are now almost always recorded digitally. To accomplish this, the flux of photons that forms the final image must be divided into small geometrical subunits called pixels. The light intensity in each pixel will be stored as a single number. Changing the objective magnification, the zoom magnification on your confocal control panel, or choosing another coupling tube magnific...

Optical Projection Tomography (OPT) is a recently developed implementation of computed tomography (CT) techniques at optical frequencies. A series of 2D optical projections through a sample are generated at varying orientations, from which the 3D structure of the sample can be computationally reconstructed. OPT is especially suitable for samples from about 0.5 mm to 15 mm in size, which fills a...

When most biomedical researchers think “confocal microscopy”, they usually have fluorescence imaging in mind. There is a very good reason for this connection. Many biomedical applications of the confocal microscope have utilized its optical sectioning power, combined with the exquisite specificity of immunofluorescence or fluorescence in situ hybridization (FISH) to produce improved images of m...

Abstract Laser distance sensors are a widespread, fast and contactless approach for surface topography measurements. Main characteristics of those given by resolution, measurement speed sensor geometry. With decreasing size, the alignment optical components in setup becomes more challenging. The depth response profilers is analyzed to obtain characteristic parameters and, thus, value transfer b...

purpose: to describe a patient with eyelid sarcoidosis. case report: a 54 year-old patient was referred for an eyelid mass from 6 months ago.excisional biopsy showed inflammatory granuloma with tuton gaint cells. chest radiograph demonstrated bilateral hilar adenopathy, increased absorption on gallium scan was noted and bronchoalveolar lavage confirmed a diagnosis of sarcoidosis. treatment with...