A method for the separation and fractionation of the major whey proteins from a whey protein concentrate (WPC80) by anion-exchange chromatography coupled to a Fast Protein Liquid Chromatography (FPLC) system is proposed. The method is based on the use of an ionic column (Mono Q) and a salt gradient elution by increasing the ionic strength of the elution buffer (Tris–HCl 20 mM plus 0 to 1 M NaCl...

The availability of deuterium-labeled nicotinic acid makes stable isotope dilution mass spectromerty (MS) coupled with liquid chromatography (LC) an attractive option for the determination of the water-soluble B-vitamin niacin in food samples. A method was developed based on acid digestion, solid-phase extraction with a strong cation exchange column, and reversed-phase chromatography with a C18...

Characterization of mercury binding protein in the human body is very important for understanding the metabolism and the mechanism of toxication of ingested mercuric compounds. In this study, mercury-containing protein in human plasma was separated by on-line heart-cutting two-dimensional high performance liquid chromatography (2D-HPLC). This 2D separation system used size exclusion liquid chro...

chamomile ( matricaria chamomilla l.) is a widely used medicinal plant possessing several pharmacological effects due to presence of active compounds. this study describes a method of using ultra performance liquid chromatography (uplc) coupled with photodiode array (pda) detector for the separation of phenolic compounds in m. chamomilla and its crude extracts. separation was conducted on c 18 ...

a fast, accurate, sensitive, selective and reliable method using reversed-phase high performance liquid chromatography coupled to electrospray ionization ion trap mass spectrometry was developed and validated for the determination of finasteride in human plasma. after protein precipitation with perchloric acid, satisfactory separation was achieved on a zorbax eclipse® c8 analytical column using ...

Zhengtian pills (ZTPs) are traditional Chinese medicine (TCM) which have been commonly used to treat headaches. Volatile components of ZTPs extracted by ethyl acetate with an ultrasonic method were analyzed by gas chromatography mass spectrometry (GC-MS). Twenty-two components were identified, accounting for 78.884% of the total components of volatile oil. The three main volatile components inc...

Supercritical uid chromatography (SFC) coupled with triple quadrupole mass spectrometry was applied to the pro ling of sucrose fatty acid esters (SEs). e SFC conditions (column and modi er gradient) were optimized for the e ective separation of SEs. In the column test, a silica gel reversed-phase column was selected. en, the method was used for the detailed characterization of commercial SEs an...

BACKGROUND Pentachloronitrobenzene (PCNB) and hexachlorobenzene (HCB) are highly toxic and widespread in every environmental compartment. Some of metabolic products such as amino/nitro containing chlorinated aromatic compounds can be determined by gas chromatography coupled with electron capture detector (GC-ECD). However, it is difficult to identify some of chlorophenolic and chloroquinolic in...