AMP + 2 phosphocreatine -+ ATP + 2 creatine. The conversion is not prevented as a result of treatment of the kinases with charcoal and Dowex 1 (CFform) to remove nucleotides. 2. It has been confirmed that neither creatine kinase nor adenylate kinase catalyses the direct phosphorylation of AMP by phosphocreatine. 3. It appears that reaction occurs because crystalline creatine kinase contains a s...

ADP is an important regulator of hepatic metabolism. Despite its importance the level of free ADP in the liver remains controversial. Recently, we engineered transgenic mice which express high levels of creatine kinase in liver. The reaction catalyzed by creatine kinase was assumed to be at equilibrium and used to calculate a free ADP level of 0.059 mumol/g wet weight. In this report we test th...

A novel immunochemical technique for a specific enzymic determination of the myocardial isoenzyme of creatine kinase, CK-MB, involves determination of B-subunit activity of a specimen in which the M-subunit activity has been inhibited by specific antibodies to the M-subunit. Interfering activities from CK-BB isoenzyme, atypical forms of creatine kinase, and adenylate kinase are eliminated by us...

OBJECTIVE To evaluate a rapid immunoturbidimetric assay for myoglobin and to investigate its clinical usefulness in the early detection of acute myocardial infarction. DESIGN Prospective study. Immunoturbidimetrically determined myoglobin concentrations were compared with radioimmunoassay results obtained with the same blood samples. The diagnostic performance of myoglobin determination was c...

Intact mitochondria from human, beef, and rat heart, as well as rat brain and rat skeletal and smooth muscle, contain considerable creatine kinase activity; those from rat or rabbit liver, kidney, and testis contain essentially none. In rat heart mitochondria creatine kinase activity is far higher than the activity of adenylate kinase and nucleoside diphosphokinase. The heart mitochondrial crea...

The reaction catalyzed by rabbit muscle creatine kinase ATP + creatine in equilibrium ADP + P-creatine has been investigated by 31P NMR. At pH 8.0 and 4 degrees C, the equilibrium constant of the overall reaction [P1][P2]/[S1] [S2] is found to be 0.08, while that for the interconversion step between enzyme-bound substrates and products [E.P1. P2]/[E.S1.S2] is estimated to be approximately 1; th...

Objective(s):In this study, we aimed at evaluation of electrophysiological and histopathalogical characteristics of statin-induced muscle injury as well as clinical features of patients who develop this condition in terms of frequency and pattern of evolution. Materials and Methods: Forty patients (age 39-74 years) including 25 subjects with type 2 diabetes mellitus, 9 with cardiovascular disea...

Objective Optimum recovery can prevent the athlete's functional capacity from falling between training sessions or competitions. The aim of this study was to evaluate effect dry cupping therapy and creatine supplementation on lactic acid, lactate dehydrogenase plasma kinase following Wingate anaerobic test in male handball players. Methods In quasi-experimental study, 12 young players 4 stages ...