The value of estimating the activities of various serum enzymes, particularly creatine kinase (CK) in confirming the diagnosis of the Duchenne type of progressive muscular dystrophy and in detecting the disease in apparently unaffected younger male sibs of known cases is well established (Chung, Morton, and Peters, 1960; Aebi, Richterich, Stillhart, Colombo, and Rossi, 1961; Pearce, Pennington,...

Metastable creatine kinase MM isoenzyme was isolated and partially purified from homogenates of myocardium and skeletal muscle by gradient elution on carboxymethyl cellulose. This variant isoenzyme migrated between the MM and MB isoenzymes on agarose electrophoresis, accounted for 3.5% of the total creatine kinase activity in each tissue, was not a macromolecule, and had stable electrophoretic ...

Background: The cardiac effects simultaneously occurring during experimental hypertension and diabetes have rarely been investigated. This study aimed at examining the effects of short-term renovascular hypertension and type 2 diabetes on cardiac functions. Methods: Five groups (7 each) of male Sprague-Dawley rats, including a control group, a diabetes (induced by Streptozocin and Nicotinamide)...

OBJECTIVE To evaluate a rapid immunoturbidimetric assay for myoglobin and to investigate its clinical usefulness in the early detection of acute myocardial infarction. DESIGN Prospective study. Immunoturbidimetrically determined myoglobin concentrations were compared with radioimmunoassay results obtained with the same blood samples. The diagnostic performance of myoglobin determination was c...

Creatine kinase (EC 2.7.3.2) B-subunit activity in serum may be routinely measured as residual activity after specific immunoinhibition of the M-subunit. We assessed the inhibition kinetics, specificity, completeness of inhibition, and inhibitory capacity of three different anti-M preparations, with use of isolated human BB, MM, and MB isoenzymes. The Scandinavian-recommended reaction system wa...

Intact mitochondria from human, beef, and rat heart, as well as rat brain and rat skeletal and smooth muscle, contain considerable creatine kinase activity; those from rat or rabbit liver, kidney, and testis contain essentially none. In rat heart mitochondria creatine kinase activity is far higher than the activity of adenylate kinase and nucleoside diphosphokinase. The heart mitochondrial crea...

Three current methods for the determination of the creatine kinase-MB activity were evaluated: electrophoresis (Beckman Inst.), ion-exchange MB-Zyme (J.T. Baker) and ion-exchange on aca (Du Pont). Eighty patients were selected out of three groups: acute myocardial infarction, heart and vascular surgery patients and a number of noncardiac cases. The precision of the ion-exchange methods was comp...

Elemental sulfur is a specific and potent inhibitor of the muscle-type isoenzyme of adenylate kinase (EC 2.7.4.3). We find inhibition by sulfur and by diadenosine pentaphosphate to be similarly potent and specific. Some properties of inhibition of adenylate kinase isoenzymes by sulfur are given. The adenylate kinase isoenzymes from skeletal muscle, brain, and heart muscle are inhibited by sulfu...

The aim of the study was to evaluate usefulness determining markers myocardial damage, such as: aspartate aminotransferase, total creatine kinase, cardiac kinase isoenzyme, lactate dehydrogenase, dehydrogenase isoenzyme and peripheral blood lactic acid in sport horses subjected physical exertion. research carried out on 50 three breeds: Ukrainian Riding, Hanoverian Westphalian, aged 4-16 years....

Interference of adenylate kinase with Oliver's method [Biochem. J. 61, 116 (1955)] for creatine kinase is usually suppressed by including an adenylate kinase inhibitor, AMP. We studied the kinetics and compared the inhibition capacities of AMP and diadenosine pentaphosphate. Both are competitive inhibitors, AMP being markedly weaker, with a Ki of about 300 mumol/liter for adenylate kinase from ...