نتایج جستجو برای: crispr associated protein 9

تعداد نتایج: 2888163  

Journal: :Annual review of biochemistry 2016
Haifeng Wang Marie La Russa Lei S Qi

The Cas9 protein (CRISPR-associated protein 9), derived from type II CRISPR (clustered regularly interspaced short palindromic repeats) bacterial immune systems, is emerging as a powerful tool for engineering the genome in diverse organisms. As an RNA-guided DNA endonuclease, Cas9 can be easily programmed to target new sites by altering its guide RNA sequence, and its development as a tool has ...

Journal: :Cell 2009
Caryn R. Hale Peng Zhao Sara Olson Michael O. Duff Brenton R. Graveley Lance Wells Rebecca M. Terns Michael P. Terns

Compelling evidence indicates that the CRISPR-Cas system protects prokaryotes from viruses and other potential genome invaders. This adaptive prokaryotic immune system arises from the clustered regularly interspaced short palindromic repeats (CRISPRs) found in prokaryotic genomes, which harbor short invader-derived sequences, and the CRISPR-associated (Cas) protein-coding genes. Here, we have i...

2016
Wei-Jing Dai Li-Yao Zhu Zhong-Yi Yan Yong Xu Qi-Long Wang Xiao-Jie Lu

Owing to its easy-to-use and multiplexing nature, the genome editing tool CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats (CRISPR) associated nuclease 9) is revolutionizing many areas of medical research and one of the most amazing areas is its gene therapy potentials. Previous explorations into the therapeutic potentials of CRISPR-Cas9 were mainly conducted in vitro or i...

Journal: :Investigative ophthalmology & visual science 2016
Sandy S C Hung Vicki Chrysostomou Fan Li Jeremiah K H Lim Jiang-Hui Wang Joseph E Powell Leilei Tu Maciej Daniszewski Camden Lo Raymond C Wong Jonathan G Crowston Alice Pébay Anna E King Bang V Bui Guei-Sheung Liu Alex W Hewitt

PURPOSE Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-associated protein (Cas) has recently been adapted to enable efficient editing of the mammalian genome, opening novel avenues for therapeutic intervention of inherited diseases. In seeking to disrupt yellow fluorescent protein (YFP) in a Thy1-YFP transgenic mouse, we assessed the feasibility of utilizing the adeno...

Journal: :The Indonesian Biomedical Journal 2022

BACKGROUND: Bone Morphogenetic Protein Receptor II (BMPR2) deficiency is associated with the pathologic development of pulmonary vascular changes in Pulmonary Arterial Hypertension (PAH). Fibroblast most abundant cell vascular. However, there only a little information regarding effect BMPR2 fibroblast. This study aims to understand fibroblasts.METHODS: applied CRISPR/Cas9 technique edit BMPPR2 ...

2016
Xuelian Wang Xiumin Huang Xiuli Fang Youzhong Zhang Wanpeng Wang

Targeted nucleases are influential instruments for intervening in genome revision with great accuracy. RNA-guided Cas9 nucleases produced from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have noticeably altered the means to modify the genomes of distinct organisms. They can be notably used to facilitate effective genome manipulation in eukaryotic cells by clea...

Journal: :Circulation. Cardiovascular genetics 2014
Rajat M Gupta

One of the most inspiring examples of rapid bench-to-bedside translation has been the development of proprotein conver-tase subtilisin/kexin 9 (PCSK9) inhibitory antibodies for the reduction of serum low-density lipoprotein and prevention of coronary artery disease. Astute genetic analysis of gain-of-function mutations in patients with elevated low-density lipo-protein (LDL) levels 1 built the ...

Journal: :Brain & development 2017
Jing-Zhang Wang Peng Wu Zhi-Min Shi Yan-Li Xu Zhi-Jun Liu

Mutations in the dystrophin gene (Dmd) result in Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD), which afflict many newborn boys. In 2016, Brain and Development published several interesting articles on DMD treatment with antisense oligonucleotide, kinase inhibitor, and prednisolone. Even more strikingly, three articles in the issue 6271 of Science in 2016 provide new ins...

Journal: :Cell 2015
Joshua Carter Blake Wiedenheft

Bacteria and archaea have evolved sophisticated adaptive immune systems that reply on CRISPR loci and a diverse cassette of Cas genes that are classified into three main types and at least eleven subtypes. All CRISPR-Cas immune systems operate through three main stages: acquisition, biogenesis, and interference. This SnapShot summarizes our current knowledge of these fascinating immune systems.

Journal: :FEMS microbiology reviews 2015
Emmanuelle Charpentier Hagen Richter John van der Oost Malcolm F White

CRISPR-Cas is an RNA-mediated adaptive immune system that defends bacteria and archaea against mobile genetic elements. Short mature CRISPR RNAs (crRNAs) are key elements in the interference step of the immune pathway. A CRISPR array composed of a series of repeats interspaced by spacer sequences acquired from invading mobile genomes is transcribed as a precursor crRNA (pre-crRNA) molecule. Thi...

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