Landfarm soil is used to bioremediate oil wastes from petrochemical industries. We developed a simplified protocol for microbial DNA extraction of tropical landfarm soil using only direct lysis of macerated material. Two samples of tropical landfarm soil from a Brazilian refinery were analyzed by this protocol (one consisted of crude oil-contaminated soil; the other was continuously enriched fo...

Controlled laboratory microcosms were used to research the phytoremediation potential of lupines (Lupinus chamissonis) for hydrocarbon-contaminated groundwater at a former oil field near Guadalupe, California. During oil production in the Guadalupe Oil Field, a kerosene-like hydrocarbon mixture was used as a diluent to improve the flow of the heavy crude oil. Leaking tanks and pipes resulted in...

We studied the effects of aged total petroleum hydrocarbons (aged TPH) on the survival of allochthonous diesel-degrading Rhodococcus sp. strain YS-7 in both laboratory and field investigations. The aged TPH extracted from a crude oil-contaminated site were fractionized by thin-layer chromatography/flame ionization detection (TLC/FID). The three fractions identified were saturated aliphatic (SA)...

Currently, the primary source of pollution is crude oil production. Crude production has dramatic consequences for farmlands, communities, and in terms construction materials required earthworks. The main aims present study were to reduce level caused by Khurmala soil then reuse it as a material. Soil remediation using solidification/stabilization method was applied field Portland limestone cem...

Approximatelly 0.6 million tons of petroleum is poured into soil, groundwater, rivers and oceans every year in China. A large amount of the oil contaminated soil has not been remediated. The contaminated soil poses a severe threat to the environment and must be taken care of. Bioremediation has become one of the most popular and promising technologies with growing demand for the remediation of ...

A solid-state bioprocess with wheat bran and rice husk as substrate was used to isolate filamentous fungi with lipase activity from dairy effluent and soil contaminated with diesel oil. The lipase activity was measured in units, with one unit (U) being defined as the amount of enzyme required to liberate 1 micromol of fatty acids per minute per gram of bran substrate (1 U = 1 micromol min(-1) g...

Bacteria able to produce biosurfactants can use petroleum-based hydrocarbons as a carbon source. Herein, four biosurfactant-producing Pseudomonas aeruginosa strains, isolated from oil-contaminated saline soil, were combined to form a bacterial consortium. The inoculation of the consortium to contaminated soil alleviated the adverse effects of salinity on biodegradation and increased the rate of...

Deliberate and accidental releases of opportunistic pathogens such as Pseudomonas aeruginosa into the environment increase the likelihood of spreading infectious diseases. Also, P. aeruginosa is responsible for nosocomial infections and usually harbour several antibiotic resistance genes. In the present study total of 76 strains of P. aeruginosa were characterized (54 strains were isolated from...

The Pseudomonas sp. P-1 strain, isolated from heavily petroleum hydrocarbon-contaminated soil, was investigated for its capability to degrade hydrocarbons and produce a biosurfactant. The strain degraded crude oil, fractions A5 and P3 of crude oil, and hexadecane (27, 39, 27 and 13% of hydrocarbons added to culture medium were degraded, respectively) but had no ability to degrade phenanthrene. ...

the removal of disulfide oil (dso) from contaminated soil was studied by the bioremediation method and the influence of electrokinetic on the bioremediation process was investigated. the bacillus subtilis strain was used in the bioremediation process. the effects of humidity, time and dso concentration in soil was studied. the experimental results for the bioremediation of dso show, the removal...