نتایج جستجو برای: ctdna

تعداد نتایج: 831  

2017
Benoit Busser Julien Lupo Lucie Sancey Stéphane Mouret Patrice Faure Joel Plumas Laurence Chaperot Marie Thérèse Leccia Jean Luc Coll Amandine Hurbin Pierre Hainaut Julie Charles

Melanoma is a cutaneous cancer with an increasing worldwide prevalence and high mortality due to unresectable or metastatic stages. Mutations in BRAF, NRAS, or KIT are present in more than 60% of melanoma cases, but a useful blood-based biomarker for the clinical monitoring of melanoma patients is still lacking. Thus, the analysis of circulating tumor cells (CTCs) and/or cell-free circulating t...

Journal: :Clinical chemistry 2016
Sonia Garrigou Geraldine Perkins Fanny Garlan Corinne Normand Audrey Didelot Delphine Le Corre Sanam Peyvandi Claire Mulot Ralph Niarra Pascaline Aucouturier Gilles Chatellier Philippe Nizard Karla Perez-Toralla Eleonora Zonta Cecile Charpy Anais Pujals Caroline Barau Olivier Bouché Jean-François Emile Denis Pezet Frederic Bibeau J Brian Hutchison Darren R Link Aziz Zaanan Pierre Laurent-Puig Iradj Sobhani Valerie Taly

BACKGROUND Circulating tumor DNA (ctDNA) has emerged as a good candidate for tracking tumor dynamics in different cancer types, potentially avoiding repeated tumor biopsies. Many different genes can be mutated within a tumor, complicating procedures for tumor monitoring, even with highly sensitive next-generation sequencing (NGS) strategies. Droplet-based digital PCR (dPCR) is a highly sensitiv...

2017
Nicolas Guibert Julien Mazieres Myriam Delaunay Anne Casanova Magali Farella Laura Keller Gilles Favre Anne Pradines

OBJECTIVES Pseudo-progression is a rare but worrying situation for both clinicians and patients during immunotherapy. Dedicated ir-RECIST criteria have been established to improve this situation. However, this can be sometimes considered inadequate and patients experiencing true progression may then receive inefficient treatments. Additional reliable tools to discriminate pseudo from true progr...

2018
Pieter A. Boonstra Arja ter Elst Marco Tibbesma Lisette J. Bosman Ron Mathijssen Florence Atrafi Frits van Coevorden Neeltje Steeghs Sheima Farag Hans Gelderblom Winette T.A. van der Graaf Ingrid M.E. Desar Jacqueline Maier Jelle Overbosch Albert J.H. Suurmeijer Jourik Gietema Ed Schuuring Anna K.L. Reyners

Background Gastrointestinal stromal tumors (GISTs) are characterized by oncogenic KIT mutations that cluster in two exon 11 hotspots. The aim of this study was to develop a single, sensitive, quantitative digital droplet PCR (ddPCR) assay for the detection of common exon 11 mutations in both GIST tumor tissue and in circulating tumor DNA (ctDNA) isolated from GIST patients' plasma. Methods A ...

2018
Nicholas C. Eastley Barbara Ottolini Rita Neumann Jin-Li Luo Robert K. Hastings Imran Khan David A. Moore Claire P. Esler Jacqueline A. Shaw Nicola J. Royle Robert U. Ashford

Following treatment 40% of soft tissue sarcoma (STS) patients suffer disease recurrence. In certain cancers circulating cell free DNA (cfDNA) and circulating tumour-derived DNA (ctDNA) characteristics correlate closely with disease burden, making them exciting potential sources of biomarkers. Despite this, the circulating nucleic acid characteristics of only 2 STS patients have been reported to...

2017
Yi-Xin Hao Qiang Fu Yan-Yan Guo Ming Ye Hui-Xia Zhao Qi Wang Xiu-Mei Peng Qiu-Wen Li Ru-Liang Wang Wen-Hua Xiao

Circulating tumor DNA (ctDNA) can be identified in the peripheral blood of patients and harbors the genomic alterations found in tumor tissues, which provides a noninvasive approach for detection of gene mutations. We conducted this meta-analysis to investigate whether ctDNA can be used for monitoring KRAS gene mutations in colorectal cancer (CRC) patients. Medline, Embase, Cochrane Library and...

2017
Xiao Han Junyun Wang Yingli Sun

Detection of circulating tumor DNAs (ctDNAs) in cancer patients is an important component of cancer precision medicine ctDNAs. Compared to the traditional physical and biochemical methods, blood-based ctDNA detection offers a non-invasive and easily accessible way for cancer diagnosis, prognostic determination, and guidance for treatment. While studies on this topic are currently underway, clin...

2017
Nithya Krishnamurthy Emily Spencer Ali Torkamani Laura Nicholson

The use of circulating tumor DNA (ctDNA) as a novel and non-invasive test for the diagnosis and surveillance of cancer is a rapidly growing area of interest, with sequencing of ctDNA acting as a potential surrogate for tissue biopsy. Circulating tumor DNA has been detected incidentally during noninvasive prenatal testing and additionally in more than 75% of known cancer patients participating i...

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه تبریز - دانشکده شیمی 1390

کمپلکس های لانتانیدی (مخصوصاً کمپلکس های تربیوم و اروپیوم) به علت داشتن خواص ویژه از جمله جابجایی استوکس زیاد، زمان زوال طولانی، حساسیت بالا، دقت خوب، پایداری خوب و گزینش پذیری نسبی می توانند برای اندازه گیری ترکیبات آلی، داروها، اسیدهای نوکلئیک، پروتئین ها و آثار یون های خاک نادر در محیط های پیچیده ی بیولوژیکی مورد استفاده قرار گیرند. در این روش لیگاند کمپلکس شده به لانتانید باعث انتقال موثر ان...

2018
Ruilian Xu Guolin Zhong Tanxiao Huang Wan He Cheng Kong Xiaoni Zhang Ying Wang Ming Liu Mingyan Xu Shifu Chen

Lung cancer is the most commonly occurring type of cancer worldwide and also has the highest mortality rate. Although targeted therapy of non-small cell lung carcinoma (NSCLC) has become common, the majority of patients receiving first-line epithelial growth factor receptor (EGFR)-TKI treatment develop drug resistance. The EGFR T790M (NM_005228.4(EGFR):c.2369C>T (p.Thr790Met)) mutation accounts...

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