The Cloud computing becomes an innovative computing paradigm, which aims to provide reliable, customized and QoS guaranteed computing infrastructures for users. This paper presents our early experience of Cloud computing based on the Cumulus project for compute centers. In this paper, we introduce the Cumulus project with its various aspects, such as design pattern, infrastructure, and middleware.

Substantially less development to the blastocyst stage occurs in vitro than in vivo and this may be due to deficiencies in oocyte competence. Although a large proportion of bovine oocytes undergo spontaneous nuclear maturation, less is known about requirements for proper cytoplasmic maturation. Commonly, supraphysiological concentrations of FSH and LH are added to maturation media to improve cu...

Polyspermy or the penetration of more than one sperm cell remains a problem during porcine in vitro fertilization (IVF). After in vitro culture of porcine zygotes, only a low percentage of blastocysts develop and their quality is inferior to that of in vivo derived blastocysts. It is unknown whether the cytoplasmic maturation of the oocyte is sufficiently sustained in current in vitro maturatio...

Assisted reproductive technologies in all mammals are critically dependent on the quality of the oocytes used to produce embryos. For reasons not fully clear, oocytes matured in vitro tend to be much less competent to become fertilized, advance to the blastocyst stage, and give rise to live young than their in vivo-produced counterparts, particularly if they are derived from immature females. H...

OBJECTIVE To test whether angiotensin II (AngII) could modulate apoptosis of human granulosa-lutein (GL) cells from gonadotropin-stimulated follicles. DESIGN In vitro assays on mural and cumulus granulosa cells. SETTING University laboratory and private IVF practice. PATIENT(S) One hundred six consecutive women undergoing 113 IVF cycles. INTERVENTION(S) Purified human GL mural or cumulu...

BACKGROUND Serum AMH is declining with age and is highly associated with ovarian follicular reserve and disordered folliculogenesis. However, the precise role of AMH in the process of human follicular aging has still to be determined. AIM This study investigates AMH level in the follicular fluid (FF) and mRNA expression pattern in cumulus and mural granulosa cells of human ovarian follicles i...

This paper prototypes a method for calibrating a cumulus parameterization against a cumulus ensemble model. The key to this technique is to run the cumulus model and the parameterization in identical test cells which provide forcing typical of that seen over tropical oceans. In particular, the mean temperature pro le is relaxed to a reference pro le which is assumed to be characteristic of the ...

background: the mitogen-activated protein kinase (mapk) pathway is one of the major signaling pathways that transmit intracellular signals initiated by extracellular stimuli to the nucleus. the stress-activated protein kinase (sapk)/c-jun nh2-terminal kinase is a subfamily of map kinases implicated in cytokine and stress responses. objective: in this study, we have examined total and phosphoryl...

Follicle-stimulating hormone (FSH) and oocyte-secreted factors influence granulosa cell differentiation and follicle development. Whereas FSH stimulates the expression of mural cell transcripts, oocyte-secreted factors regulate specific cumulus cell genes and suppress the appearance of mural cell transcripts. This study addresses the extent to which clinically relevant changes in FSH doses appl...

PURPOSE Cumulus cells have a critical role in normal oocyte development and fertilization. Prunus cerasus is an anthocyanin rich berry and performs strong antioxidant activity. The present study set to determine if Prunus cerasus can affect expression of HAS2 (hyaluronan synthase 2) and progesterone receptor in Cumulus cells and its consequences outcome of the in vitro fertilization. METHODS ...