As a crucial component in TLR/IL-1R signaling pathways, IRAK-4 plays a central role in innate and adaptive immunity. In the present study, the cDNA of IRAK-4 was cloned for the first time from half-smooth tongue sole (Cynoglossus semilaevis). The full-length cDNA of csIRAK-4 was 2149 bp and contained a 168 bp 5' UTR, a 580 bp 3' UTR and a 1401 bp CDS. The predicted protein sequence of csIRAK-4 ...

Half-smooth tongue sole (Cynoglossus semilaevis) is one of the most important flatfish species for aquaculture in China. To produce a monosex population, we attempted to develop a marker-assisted sex control technique in this sexually size dimorphic fish. In this study, we identified a co-dominant sex-linked marker (i.e., CyseSLM) by screening genomic microsatellites and further developed a nov...

Wnt4 gene plays a role in developmental processes in mammals. However, little is known regarding its function in teleosts. We cloned and characterized the full-length half-smooth tongue sole (Cynoglossus semilaevis) wnt4a gene (CS-wnt4a). CS-wnt4a cDNA was 1746 bp in length encoding 353aa. CS-wnt4a expression level was highest in the testis, and gradually increased in the developing gonads unti...

We investigated the genetic diversity of the southern flounder Paralichthys lethostigma. Microsatellite-enriched libraries were constructed and novel microsatellite markers were developed and applied for genetic detection of wild populations. Cross-species amplification was also conducted in five pleuronectiforme species. Of 45 randomly selected and sequenced clones, 43 contained a CA or GA rep...

This study was aimed to estimate the mycoflora and aflatoxin contamination of smoked dried fishes of Stock (Gadus morhua), Skip jack tuna (Katsuworus pelamis), Croaker (Pseudotolithus typhus), Sting ray (Dasyatis margarita), Cat (Arius hendeloti), Bonga (Ethalmosa fimbriota), Ribban fish (Triuchurius trichurius), Stark (Carchanas faunis), Thread fin (Pentanemis qumquarius), Sole (Cynoglossus br...

NK-lysin is an antimicrobial protein produced by cytotoxic T lymphocytes and natural killer cells. In this study, we examined the biological property of a peptide, NKLP27, derived from tongue sole (Cynoglossus semilaevis) NK-lysin. NKLP27 is composed of 27 amino acids and shares little sequence identity with known NK-lysin peptides. NKLP27 possesses bactericidal activity against both Gram-negat...

Deep learning has become the state-of-art tool in many applications, but the evaluation and training of deep models can be time-consuming and computationally expensive. The conditional computation approach has been proposed to tackle this problem (Bengio et al., 2013; Davis & Arel, 2013). It operates by selectively activating only parts of the network at a time. In this paper, we use reinforcem...

The Hierarchical Temporal Memory (HTM) approach to perception processing, pursued by prior authors including Jeff Hawkins and Itamar Arel, is expanded to encompass more abstract semantic forms of HTM, and to include separate, coupled hierarchies for perception, motor control and goal management. No experimental evidence or detailed calculations are presented; this is merely a conceptual paper d...