an investigation was carried out to reveal the changes in the concentration of soluble carbohydrates under tuber dormancy in food yam (dioscorea esculenta) tubers and in an economically important spice cum medicinal plant turmeric (curcuma longa) rhizomes under storage. harvested, fully matured tubers of yam and rhizomes of turmeric were stored in wooden boxes under the conditions of 28 ? 2?c t...

one of the most effective ways to propagate lilium is by using tissue culture techniques, preferably through bulblet production. in addition to the surviving percentage of lilium bulbs after transplantation, the small size of in vitro bulblets compared to commercial bulbs and bulblets‟ dormancy are the most important constraints to commercializing lilium micropropagation. different concentratio...

Page Page Acknowledgments ......................................................................................... 2 Time of Initiating Dietary Fat Supplementation on Lactation and Reproduction .................................................................................. 3 A Soyhull:Soy Lecithin:Soapstock Mixture for Early Lactation Dairy Cows ................................................

This case report describes intracytoplasmic crystalline inclusions in the hepatocytes of a 13-year-old female Thomson's gazelle. Histologically, multifocal to coalescing areas of many hepatocytes contained large cytoplasmic vacuoles filled with pale eosinophilic homogeneous material and rare fine basophilic granules. Von Kossa staining showed the presence of calcium within cytoplasm, mainly in ...

The ability of Salmonella to cause disease depends on metabolic activities and virulence factors. Here, we show that a key metabolic protein, EIIAGlc, is absolutely essential for acute infection, but not for Salmonella survival, in a mouse typhoid fever model. Surprisingly, phosphorylation-dependent EIIAGlc functions, including carbohydrate transport and activation of adenylate cyclase for glob...

With procedures developed previously in this laboratory, the various layers of the cell wall of a gram-negative bacterium, a marine pseudomonad (ATCC 19855), were removed completely giving rise to true protoplasts. Membranes were isolated from the protoplasts formed. After treatment with ribonuclease, deoxyribonuclease, and washing, the membranes isolated were shown by electron microscopy and c...

Large polylactosaminoglycans have only been observed linked to membrane proteins. To determine if membrane anchoring of a secretory protein might lead to the addition of polylactosaminoglycan, we have examined the carbohydrate structure on a membrane-anchored form of human chorionic gonadotropin-alpha subunit. This protein was generated by fusing the DNA encoding the human chorionic gonadotropi...

We identified a new human, T-lineage restricted glycoprotein of molecular weight 120 Kd that is expressed primarily in mature T-cell malignancies. The antigen, named TCA-1 (T-cell cytoplasmic antigen), is an intracellular glycoprotein found mainly in the Golgi stacks, although a few cell lines also display surface membrane TCA-1. Many but not all T-cell neoplasms express this antigen. The antig...

Cytoplasmic dynein is responsible for intra-cellular transport in eukaryotic cells. Using Fluctuating Finite Element Analysis (FFEA), a novel algorithm that represents proteins as continuum viscoelastic solids subject to thermal noise, we are building computational tools study the mechanics of these molecular machines. Here present methodology obtaining material parameters required represent fl...

The cell wall of Sulfolobus acidocaldarius has been isolated. Cells were mechanically disrupted with a French press, and the cytoplasmic membrane was removed by extracting cell-envelope fragments with Triton X-100. The Triton-insoluble cell wall material retained the characteristic subunit structure when examined in the electron microscope. Isolated cell wall fragments formed in open sheets tha...