this study was conducted to evaluate the effects of in ovo feeding of different nutrients on hatchability, initial weight after hatch, growth performance, blood parameters, immune organs and intestinal morphology of broiler chicks. the experiment was conducted in a completely randomized design with 5 treatments, 3 replicates and 16 fertile eggs from ross 308 breeders (28 week old) in each repli...

The proximate composition, amino acids and fatty acids composition in the muscle of wild, inshore and offshore cage-cultured large yellow croaker, Pseudosciaena crocea (Richardson, 1846), were determined to identify nutritional differences. Wild fish groups showed highest content of moisture and crude protein, but the lowest lipid content. Offshore cage-cultured fish showed significantly higher...

The structure of a unique polythiazole-containing cyclic peptide antibiotic, amythiamicin D, was elucidated by chemical degradations and NMR spectral analyses. Acid hydrolysis of amythiamicin D gave one mole of glycine and three new amino acids. Structures of N-acetyl-O-methyl derivatives of these new amino acids were determined by NMR and UV spectral analyses. Connectivities of these amino aci...

Isoelectric solubilization /precipitation (ISP) process was used to isolate protein from muscles of Prussian carp, Carassius gibelio (Bloch, 1782). Fish protein and lipid were recovered from whole gutted Prussian carp using acidic and basic isoelectric solubilization/precipitation followed by assaying amino acid and fatty acid profile. Essential amino acids content in acidic and basic pH treatm...

DTD (D-Tyr-tRNA(Tyr) deacylase) is known to be able to deacylate D-aminoacyl-tRNAs into free D-amino acids and tRNAs and therefore contributes to cellular resistance against D-amino acids in Escherichia coli and yeast. We have found that h-DTD (human DTD) is enriched in the nuclear envelope region of mammalian cells. Treatment of HeLa cells with D-Tyr resulted in nuclear accumulation of tRNA(Ty...

Screen-printed three-electrode amperometric sensors incorporating L- and/or D-amino acid oxidase for the general purpose measurement of L- or D-amino acids is described. The working electrode incorporates rhodinized carbon, to facilitate hydrogen peroxide oxidation at a decreased operating potential, and immobilized enzyme. The devices responded to all 20 common L-amino acids and all of the D-a...

d-Amino acids are now recognized to be widely present in mammals. Renal d-amino-acid oxidase (DAO) is associated with conversion of d-amino acids to the corresponding alpha-keto acids, but its contribution in vivo is poorly understood because the alpha-keto acids and/or l-amino acids formed are indistinguishable from endogenous compounds. First, we examined whether DAO is indispensable for conv...

All amino acids except glycine have stereoisomers (Land D-forms). Earlier studies indicate that only L-amino acids are present in higher organisms, such as mammals. However, owing to progress in analytical methods, numerous researchers have recently demonstrated the existence of various naturally occurring D-amino acids in mammals, such as Dserine and D-aspartate (D-Asp). Free D-Asp is present ...

the impact of pgf2? on total proteins, free amino acids and the amino transferases in the gonads of silkworm, bombyx mori l. were investigated in the present experiment. the effect of pgf2? resulted in increase in the total protein and free amino acid contents in the gonads of silkworm larvae indicating improved structural and dynamic levels of organization. the enhanced activity levels of amin...

Quorum sensing controls the luminescence of Vibrio fischeri through the transcriptional activator LuxR and the specific autoinducer signal produced by luxI. Amino acid sequences of these two genes were analyzed using bioinformatics tools. LuxI consists of 193 amino acids and appears to contain five α-helices and six ß-sheets when analyzed by SSpro8. LuxI belongs to the autoinducer synthetase fa...