The isolation of human genomic DNA is an important step involved in genetic identity analysis. Also getting optimal quantity and quality of DNA is important as this can alter the results. This consideration is especially important in the field of forensic analysis and genetic identity, where DNA is extracted from extremely small amounts of starting material often recovered from suboptimal stora...

background: the use of electric current during application of etch-and-rinse adhesive systems has been recently introduced to decrease microleakage. this study investigated the effects of an electric field produced by an experimental device for the application of a two-step etch-and-rinse adhesive on moist dentin surface. methods: sixty freshly extracted human premolars were used for this study...

INTRODUCTION Dental pulp has neural fibers that produce neuropeptides like Substance P (SP) and calcitonin gene-related peptide (CGRP). The inflammation of dental pulp can lead to an increase amount of SP and CGRP release, especially in symptomatic irreversible pulpitis. Therefore, it can be assumed that neuropeptides have some role in the progression of inflammation of the dental pulp. The aim...

A Consensus Conference on Terminology was convened by the American Association of Endodontists in Chicago on Oct 3, 2008 to review solicited papers on focused questions. This paper addressed the question: Identify and determine the metrics, hierarchy, and predictive value of all the parameters and/or methods used during endodontic diagnosis. The best available clinical evidence was used to dete...

A routine cavity preparation of a tooth may lead to opening the pulp chamber. The present study evaluates quantitatively, in real time, for the first time to the best of our knowledge, the drilled cavities during dental procedures. An established noninvasive imaging technique, Optical Coherence Tomography (OCT), is used. The main scope is to prevent accidental openings of the dental pulp chambe...

INTRODUCTION Microarray is a recently developed simultaneous analysis of expression patterns of thousand of genes. The aim of this research was to evaluate the expression profile of human healthy dental pulp in order to find the presence of genes activated and encoding for proteins involved in the physiological process of human dental pulp. We report data obtained by analyzing expression profil...

introduction: open-apex teeth with irreversible pulpitis require complex and difficult treatment. providing the right environment for apexogenesis and pulp protection is vital for their long term prognosis. the young pulp of open apex tooth, however, is better equipped against irritation and assault. the aim of this study was to evaluate pulp inflammation in open apex canine teeth of cats. mate...

introduction: knowledge of the anatomic location and dimension of the molar pulp chamber may preserve healthy pulp during operative procedure and reduce risk of perforation of chamber during access preparation. a review of literatures regarding the morphology of pulp chamber however revealed very little information; so the aim of this in vivo study was to measure the dimensions of first molar p...

introduction: ca(oh) 2 -containing/forming materials are conventionally used for indirect pulp-capping and are theoretically able to release ca 2+ and oh - ions for hydrolytic dissociation. however, no evidence exists for ion diffusion through the remaining coronal dentin. the aim of this study was to design an innovative experimental set-up to test the ability of ca(oh) 2 -containing and ca(oh...

background: managing dental caries in young children is demanding due to the elusions present on the right diagnostic criteria for treatment. the present study evaluated the histological status of pulp tissues extracted from primary second molar with caries involvement. histological findings are correlated with clinical and radiographic assessment. materials and methods: simple experimental stu...