The objective of this study was to determine the effect of medroxyprogesterone acetate (DMPA) on the development and maintenance of Candida albicans in the vagina of oophorectomized Wistar rats. The animals were divided into negative control groups (NCG), which received injections of sterile saline; positive control groups (PCG), which were given injections of estradiol valerate; and progestero...

The objective of these experiments was to determine if a change in the ratio of acetate to propionate (A:P ratio) in hepatic portal blood, draining the gastrointestinal tract, can alter the metabolism of progesterone by the liver. Experiment 1; 4 crossbred, ewe lambs (BW 45.5 + 2.5 kg) were fed for maintenance and given a once daily oral bolus (0.146 Mcal/d) of acetate (0.7 moles) or propionate...

Two enzymes are responsible for the cleavage of pregnane side chains. A steroid-inducible oxygenase, in the presence of reduced nicotinamide adenine trinucleotide and molecular oxygen, catalyzes the conversion of progesterone into testosterone acetate. The latter ester is then hydrolyzed by an esterase to yield testosterone. The oxygenase has been partially purified and separated from the ester...

Relatively little is known concerning the main tenance of biochemical integrity of tissue after it has been heterologously transplanted. The pos sibility of tissue enzyme adaptation to the new host following such a transplant is not easily ruled out. This subject is of particular interest in view of the use of human tumor transplants in rodents as a screening method for antitumor agents. A rece...

We report the case of a young woman with tuberous sclerosis who developed a chylous pleural effusion after several invasive procedures for treatment of recurring pneumothoraces. Oophorectomy was rejected by the patient and progesterone therapy initiated. Medroxyprogesterone acetate administration led to a complete disappearance of the chylothorax in 8 months. The patient was kept on therapy, an...

The most frequently identified benign tumor in women is represented by the uterine fibroids with various symptoms: metrorrhagia, dysmenorrhea, anemia, pelvic pain. Nowadays because none of the approved medical treatments has proven able to completely eliminate uterine fibroids, surgery represents the main treatment strategy. Due to its contribution in the growth of leiomyomas, progesterone play...

Nuclear transport, phosphorylation, ligand binding, and degradation rate of the recombinant androgen receptor (AR) were analyzed in transfected COS cells in the presence of various steroids and antiandrogens. Transcriptional activation was assessed in CV1 cells by cotransfection with an androgen-responsive chloramphenicol acetyltransferase (CAT) reporter vector. Hormone binding specificity of r...

Erythema nodosum is a septal panniculitis that typically presents as symmetric, tender nodules on the anterior aspects of bilateral lower extremities. Nearly half of cases are due to secondary causes, with oral contraceptive pills being the leading pharmaceutical cause. However, to our knowledge, there has yet to be a published association with norethindrone acetate, ethinyl estradiol, and ferr...

Estradiol dehydrogenase (E2DH) is a well-known progester one-dependent enzyme in human endometrium, and its induc tion has been proposed as a means to test hormonal sensitivity of endometrial carcinoma. While administration of progestins to some patients with endometrial carcinoma resulted in in creased endometrial E2DH activity, efforts to induce this en zyme, in vitro, in these tumors have be...

Free radical-generated F(2alpha)-isoprostanes are a group of compounds with vasoconstrictor properties. To investigate whether estradiol exerts antioxidant actions modifying F(2alpha)-isoprostane production, cultured human umbilical vein endothelial cells were exposed to estradiol and other compounds and F(2alpha)-isoprostanes were measured in culture medium. Exposure to 1 and 10 nM estradiol f...