The knowledge of solid loss kinetics of foods during digestion is crucial for understanding the factors that constrain the release of nutrients from the food matrix and their fate of digestion. The objective of this study was to investigate the solid loss of carrots during simulated gastric digestion as affected by pH, temperature, viscosity of gastric fluids, mechanical force present in stomac...

BACKGROUND Older individuals generally experience a reduced food-chewing efficiency. As a consequence, food texture may represent an important factor that modulates dietary protein digestion and absorption kinetics and the subsequent postprandial protein balance. OBJECTIVE We assessed the effect of meat texture on the dietary protein digestion rate, amino acid availability, and subsequent pos...

BACKGROUND AND AIMS The egg protein ovalbumin (OVA) belongs to six most frequent food allergens. We investigated how thermal processing influences its ability to induce allergic symptoms and immune responses in mouse model of food allergy. METHODOLOGY/PRINCIPAL FINDINGS Effect of increased temperature (70°C and 95°C) on OVA secondary structure was characterized by circular dichroism and by th...

Proteolysis with proteases preloaded within nanopores of porous material is a very fast process, where proteins can be digested in minutes compared to the conventional bulk enzyme reactions taking place over hours. To model this surprising phenomenon, a modified sequential proteolytic mechanism has been developed to simulate the kinetics of the reaction. Digestion of myoglobin was used as an ex...

Native cuticle collagen, obtained from Xereis virens, was incubated with purified bacterial collagenase (EC 3.4.4.19). The kinetics of proteolysis were monitored by viscometry, in parallel with similar digestions of calf skin collagen. Comparison of the kinetics of digestion of the two collagens, at similar enzyme to substrate ratios (w/w), showed that the native cuticle collagen was relatively...

Biochars made from brewer’s spent grain were added to the anaerobic digestion of enhance methane fermentation process and improve biogas production. In research, effect biochars at 300, 450, 600 °C doses 1-8% was tested. The biochemical potential tests in mesophilic conditions performed. took 28 days, yield for each reactor varied 500-650 ml×gVS-1, around 60% substrate degradation obtained. For...

Even though the availability of dietary amino acids is the result of integrated phenomena of digestion, absorption and transport, it may be mainly affected by the stage of luminal digestion. In this case, amino acid availability could be predicted by an in vitro method designed to reproduce in vivo proteolysis conditions. In order to check this hypothesis, the essential amino acid (EAA) profile...

It has been proven that the biocenosis of microalgae and bacteria improves chemical properties biomass for its use in anaerobic digestion. However, this digestion can be limited by strong, compact, complex structure granulated biomass. Therefore, there is a need to search an effective method microalgal–bacterial granular sludge pretreatment, which not undertaken previous scientific works. In st...

Lignocellulosic biomass is a potential source of renewable, low-carbon-footprint liquid fuels. Biomass recalcitrance and enzyme cost are key challenges associated with the large-scale production of cellulosic fuel. Kinetic modeling of enzymatic cellulose digestion has been complicated by the heterogeneous nature of the substrate and by the fact that a true steady state cannot be attained. We pr...

Anaerobic digestion model No.1 (ADM1) was applied and expanded in this study to model and simulate anaerobic digestion (AD) of an industrial carbon capture reclaimer MEA (monoethanolamine) waste (MEAw) together with easily degradable organics. The general structure of ADM1 was not changed except for introducing state variables of MEA and complex organics (CO) in the waste and biochemical reacti...