Pectinases are a group of enzymes with broad application, including in plant fiber processing, pectic wastewater treatment, paper pulping, fruit juice extraction, and clarification. With an increasing industrial demand for these enzymes, it is useful to isolate organisms that produce large amounts pectinase possess wide ranges stability factors like temperature pH. In this study, 17 out 29 bact...

The arginine-rich RNA-binding domain of bovine immunodeficiency virus (BIV) Tat adopts a beta-hairpin conformation upon binding to the major groove of BIV TAR. Based on its NMR structure, we modeled dimeric arrangements in which two adjacent TAR sites might be recognized with high affinity by a dimeric peptide. Some dimeric RNAs efficiently bound two unlinked BIV Tat peptides in vitro, but coul...

Wild-type recombinant horseradish peroxidase purified and refolded from Escherichia coli inclusion bodies has been studied in the system of bis(2-ethylhexyl)sulphosuccinate sodium salt (Aerosol OT)-reversed micelles in octane. In contrast with native horseradish peroxidase the wild-type recombinant enzyme forms dimeric structures as judged by sedimentation analysis. Peroxidase substrates affect...

Jamun (Syzygium cuminii L.) is one of the important indigenous minor fruit with high medicinal value. The jamun cultivation is unorganized and there is huge loss of this fruit every year. The perishable nature of the fruit makes its postharvest management further difficult. Due to the strong cell wall structure of pectin-protein bonds and hard seeds, extraction of juice becomes difficult. Enzym...

BACKGROUND Ginsenosides are the principal components responsible for the pharmacological activities of ginseng. Ginsenosides Rg1 and Rb1 are the major compounds recognized as marker substances for quality control of ginseng-based products. These major compounds can be transformed to several pharmacologically active minor ginsenosides by chemical, microbial, and enzymatic means. MATERIALS AND ...

Pectinases (EC 3.2.1.15) are a class of enzymes that catalyze the depolymerization or de-esterification reactions degrade pectic substances. In present study, we have isolated Aspergillus niger strain from soil samples, in India and evaluated pectinase production. The highest producing A. was further optimized with various agricultural wastes. Plackett-Burman design (PBD) Central composite ‘(CC...

Abstract. Utami AP, Fahrurrozi, Meryandini A. 2022. Production and immobilization pectinase from Bacillus sp. 2P11 using alginate beads. Biodiversitas 23: 3960-3966. Pectinase is one of the enzymes often used in industrial sector, especially food industry, such as extracting clarifying juices. Previous studies have obtained pectinolytic microbial cultures isolated cocoa beans. production pure p...

In this study, complete purification, characterization, and immobilization of polygalacturonase (pectinase) from Penicillium notatum were carried out to achieve an economical suitable alternative for industrial fruit juice clarification. Biosynthesis was under pre-optimized conditions employing solid-state fermentation at a pilot scale using wheat bran. The enzyme subjected series steps purific...