Two kanamycin-resistant variants of Nicotiana tabacum were derived by culturing seedling leaf sections on a shoot-inducing medium containing kanamycin. The variants displayed a higher resistance to the structurally related antibiotic streptomycin than to kanamycin. The resistance phenotype was expressed when the tissue was cultured either as callus or as a differentiating tissue and was stably ...

Until now numbers of efforts have been made to deduce the optimized conditions for the in vitro regeneration of tomato plant. The optimized conditions deduced are contradictory to each other and to validate the optimum conditions the present investigation was carried out which directs the use of 1.5 mg/l IAA + 1.5 mg/l Zeatin with full strength Murashige and Skoog’s (MS) medium for the developm...

Cotyledonary nodes taken alongwith shoot apex from seedlings of cotton (G. hirsutum) proliferated into shoots on nutrient agar medium supplemented with cytokinins. In the presence of optimal plant growth regulators, low light intensity enhanced the number of shoots initiated per explant in cotton. An average of 33.5 +/- 2.9 shoots were obtained from a single explant cultured for 8 weeks which i...

An effi cient protocol for organogenesis through leaves has been established for Launaea sarmentosa (Willd.) Sch. Bip. ex Kuntze, a highly valuable medicinal plant. The leaf explants produced microshoots on MS basal medium when fortifi ed with cytokinins and auxins. A combination of 6-benzylaminopurine (BAP) at 0.5mg/l and naphthaleneacetic acid (NAA) at 0.2mg/l resulted in the induction of hig...

In vitro organogenesis may provide a method to reduce the very large number of observations required for in planta studies on seed set in Fagopyrum esculentum. Plants were regenerated from leaf petiole explant tissue of buckwheat seedlings. Maximum shoot regeneration was induced on Murashige and Skoog (MS) medium containing Gamborg’s vitamins, 6-benzylaminopurine (BA), 6, -dimethylallylamino-pu...

shoot tips from actively growing, greenhouse maintained plants of kalanchoe blossfeldiana were cultured in vitro for shoot proliferation and root initiation on murashige and skoog (ms) basal medium supplemented with naa and ba, both in concentrations of 0.00, 0.50, 1.00 and 2.00 mg l-1. results showed that the maximum plantlets height (7.012 cm), node number (4.516), root number (8.860) and roo...

Aconitum vilmorinianum, a well-known traditional Chinese herb, is recently being threatened by overexploitation and environment disturbance. This study was conducted to provide propagation methods through in vitro germination explant cultivation. Germination stimulated up 66.00% on Murashige Skoog (MS) medium containing 2.0 mg L−1 6-benzylaminopurine (BAP), 0.1 1-napthaleneacetic acid (NAA), 30...

Abstract Lactuca indica L. (Asteraceae), a wild lettuce, is used as vegetable and in traditional medicine. This study aims to establish vitro propagation protocol evaluate lactucin antibacterial property from natural plant tissues. Leaf blades petioles were cultured on Murashige Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP) 1.2 mg L –1 indoleacetic acid...