Disulfide forms of homocysteine account for >98% of total homocysteine in plasma from healthy individuals. We recently reported that homocysteine reacts with albumin-Cys(34)-S-S-cysteine to form homocysteine-cysteine mixed disulfide and albumin-Cys(34) thiolate anion. The latter then reacts with homocystine or homocysteine-cysteine mixed disulfide to form albumin-bound homocysteine (Sengupta, S...

In the major pathway for protein disulfide-bond formation in the endoplasmic reticulum (ER), oxidizing equivalents flow from the conserved ER-membrane protein Ero1p to secretory proteins via protein disulfide isomerase (PDI). Herein, a mutational analysis of the yeast ERO1 gene identifies two pairs of conserved cysteines likely to form redox-active disulfide bonds in Ero1p. Cys100, Cys105, Cys3...

Chloroplast fructose 1,6-bisphosphatase (FBPase) is activated by reduction of a regulatory disulfide through thioredoxin f (Trx f). In the course of this reduction a transient mixed disulfide is formed linking covalently Trx f with FBPase, which possesses three Cys on a loop structure, two of them forming the redox-active disulfide bridge. The goal of this study was to identify the Cys involved...

A method for determining the kinetic fate of structured disulfide species (i.e., whether they are preferentially oxidized or reshuffle back to an unstructured disulfide species) is introduced. The method relies on the sensitivity of unstructured disulfide species to low concentrations of reducing agents. Because a structured des species that preferentially reshuffles generally first rearranges ...

—The disulfide bonds in γ-46 gliadin were identified: Cys 173 –Cys 192 , Cys 212 –Cys 291 , Cys 165 –Cys 199 (or Cys 200 ), Cys 283 –Cys 200 (or Cys 199 ). The disulfide-containing peptides were obtained by limited hydrolysis of the intact protein with chymotrypsin at an enzyme/substrate ratio of 1:1000 at 20°C for 22 h with subsequent digestion of disulfide-containing fragments with trypsin an...

A novel immobilized N-chlorosuccinimide resin was developed for peptide disulfide bond formation in combinatorial libraries. The resin is prepared in a simple two-step process from commercial starting materials. Disulfide formation is initiated by adding a peptide solution to the resin, and excess reagent is removed by a convenient filtration upon completion of disulfide formation. Completion o...

Molybdenum disulfide (MoS2) is used as a solid lubricant and well known for its tribological behavior (friction wear). The properties of the lubricating oil–MoS2 nanoparticles mixture in different conditions friction are studied using four-ball tribometer, operating four balls when immersed can be modeled. current paper presents calculating method critical sliding velocity (?cr) maximum torque ...

Durand, Kirt Lenroy. Ph.D., Purdue University, December 2014. Determination of Disulfide Bond Connecting Patterns via Tandem Mass Spectrometry (MS) and Biomolecular Ion/Radical Reactions. Major Professor: Yu Xia. Disulfide bond formation is one of the most common post translational modifications to occur in proteins and naturally occurring peptides. Disulfide bond formation plays a critical rol...

Abstract This review accumulates information from various research works about the preferable manufacturing process, process parameters, parts of compressor, working principle, material, and critical issues with some solutions that might be possible in era future industry. In addition, work is also done on tribological trends compressor. this paper, detailed has been gathered compressor their p...

A liquid chromatography tandem-mass spectrometry method was developed to map the eleven disulfide bonds in Pfs25, a malaria transmission-blocking vaccine candidate. The compact and complex nature of Pfs25 has led to difficulties in prior peptide mapping efforts. Here, we report confirmation of proper disulfide pairing of a recombinant Pfs25, by optimizing denaturation and digestion with trypsin...