Mitochondrial (mt) nucleoids were isolated with a high degree of purity from the yeast Pichia judinii, in which the mitochondrial DNA (mtDNA) is linear. Field-inversion gel electrophoresis (FIGE) reyealed that significant amounts of mtDNA could be isolated intact, as linear molecules of 41 kbp, from the isolated mt-nucleoids. Fifteen different proteins were detected in the mt-nucleoid fraction ...

OBJECTIVE The aim of this study was to evaluate, by PCR-RFLP and real-time PCR, the yield and quality of genomic DNA collected from buccal cells by mouthwash after different storage times at room temperature. MATERIAL AND METHODS A group of volunteers was recruited to collect buccal cells using a mouthwash solution. The collected solution was divided into 3 tubes, one tube were used for immed...

The selection of a DNA extraction method is a critical step when subsequent analysis depends on the DNA quality and quantity. Unlike mammals, for which several capable DNA extraction methods have been developed, for molluscs the availability of optimized genomic DNA extraction protocols is clearly insufficient. Several aspects such as animal physiology, the type (e.g., adductor muscle or gills)...

In our experimental studies of DNA hybridization as a function of fishing (exposure) time and the subsequent dissociation of bound oligomers, we compared the behaviors of two syntheses of the 59 mer target (labeled 59A and 59B) and 10 mer ODN (labeled 10A and 10B) in different experiments. The two syntheses yield significantly different results. For instance, the fraction of detectable hybridiz...

Aim: This study aimed to verify the quantity and purity of DNA obtained from buccal cells under differentstorage conditions. Methods: Thirty students, between 18 23 years age participated in study. Three samples genetic material were collected each student (samples A, B, C) through a mouth rinse with 5 mL 3% glucose. The first phase extraction sample A was carried out on same day collection, wh...

BACKGROUND Fetal DNA in maternal plasma and serum has been shown to be a useful material for prenatal fetal sex determination during early gestational ages. Non-invasive prenatal diagnosis is now possible at 8(th) week of pregnancy, by maternal blood sample testing. OBJECTIVE The purpose of this study was to evaluate two DNA extraction methods from mother plasma and its routine clinical appli...

The rapid adoption of microbial whole genome sequencing in public health, clinical testing, and forensic laboratories requires the use of validated measurement processes. Well-characterized, homogeneous, and stable microbial genomic reference materials can be used to evaluate measurement processes, improving confidence in microbial whole genome sequencing results. We have developed a reproducib...

We describe the construction and analysis of recombinant DNA libraries representative of chromosomes 1 and 2 of Chinese hamster (Cricetulus griseus). Propidium-iodide stained chromosomes were purified by flow cytometric analysis and sorting, and EcoRI digests of purified DNA were cloned into the bacteriophage vector Charon 4A. These libraries contain DNA complementary to 63% and 69% of nick-tra...