A new DNA biosensor microsystem based on nanomechanical transducers is being developed. The microsystem comprises an array of 20 cantilevers, a microfludic system for delivery of the samples, an array of 20 lasers (VSCELs) and chips with the photodetectors and the CMOS circuitry for signal acquisition and conditioning. Robust immobilization procedures for the oligonucleotides receptor sequences...

Paper-based DNA biosensors are powerful tools in point-of-care diagnostics since they affordable, portable, user-friendly, rapid and robust. However, their sensitivity is not always as high required to enable quantification. To improve the response of standard dot blots, we have applied a new enhancement strategy that increases assays based on use biotinylated silica-nanoparticles (biotin-Si-NP...

In contrast to DNA microarrays, production of protein microarrays is an immense technological challenge due to high complexity and diversity of proteins. In this paper we investigate three essential aspects of protein microarray fabrication based on the highly parallel and non-contact TopSpot technology: evaporation of probes during long lasting production times, optimization of protein immobil...

A DNA hybridization sensor using pentacene thin film transistors (TFTs) is an excellent candidate for disposable sensor applications due to their low-cost fabrication process and fast detection. We fabricated pentacene TFTs on glass substrate for the sensing of DNA hybridization. The ss-DNA (polyA/polyT) or ds-DNA (polyA/polyT hybrid) were immobilized directly on the surface of the pentacene, p...

Over the last few years, laterally microstructured arrays of DNA and protein probes have been developed as tools for high-throughput experimentation in biomedical research. These devices have the advantage of spatial addressability of the probes and require only small amounts of analyte. The development of protein microarrays is still obstructed by the intrinsic instability of many proteins, wh...

Electrochemical DNA biosensors are used widely due to its sensitivity, accessibility and accuracy. This electrochemical era in DNA biosensors took almost three decades for its development and application. Purine bases in the DNA namely guanine and adenine oxidizes at the working electrode during application of a positive potential. This property of DNA has been utilized in electrochemical DNA b...

We report on the microarray-based in vitro evaluation of two libraries of DNA oligonucleotide sequences, designed in silico for applications in supramolecular self-assembly, such as DNA computing and DNA-based nanosciences. In this first study which is devoted to the comparison of sequence motif properties theoretically predicted with their performance in real-life, the DNA-directed immobilizat...