In this paper we present a novel microfluidic platform for DNA sequencing-by-synthesis methods (e.g. pyrosequencing). The proposed platform is based on the valve-controllable PDMS channel technology with DNA-coated magnetic beads. The encapsulation of the reaction of DNA polymerization in picoliter-sized wells provides for excellent isolation and control for detection. This separation prevents ...

The traditional Sanger method of DNA sequencing, which uses dideoxynucleotides (ddNTPs) to terminate DNA synthesis at each of the four bases, has remained the gold standard in DNA sequencing for years. The recent development of ‘‘sequencing by synthesis’’ (SBS) techniques, which identify each base as the DNA strand is extended, has offered faster and cheaper sequencing in a massively parallel s...