Two-dimensional electrophoresis, with isoelectric focusing in the first dimension and sodium dodecyl sulfate/polyacrylamide gel electrophoresis in the second, has been adapted for the high-resolution analysis of cerebrospinal fluid proteins. Proteins were detected with a new, highly sensitive silver stain that made visible more than 300 polypeptides from 60 microL of spinal fluid, in highly rep...

Amphoteric azo dyes were used for internal control of pI values in Comparative two-dimensional Fluorescence Gel Electrophoresis (CoFGE) [1]. The 2D-gel images of separated Escherichia coli proteins as well as those of colored amphoteric dyes separated by isoelectric focussing are presented. The latter were used to correct for variation in the first electrophoretic dimension and further improve ...

A 58-year-old Hispanic man under treatment for a gangrenous toe was found to have chronic microcytic anemia and a positive sickle cell screen. High-performance liquid chromatography and isoelectric focusing electrophoresis showed that the patient is double heterozygous for hemoglobin S and hemoglobin Fannin-Lubbock. The patient does not have any manifestations of a sickling disorder. Capillary ...

A method is described for estimating the size of large circular DNAs found within complex chromosomal DNA preparations. DNAs are treated with low levels of gamma-irradiation, sufficient to introduce a single double-stranded break per circle, and the resulting linear DNA is sized by pulsed-field electrophoresis and blot hybridization. The method is fast, reproducible, and very conveniently appli...

Estradiol (E2) facilitates the lordosis reflex that occurs in response to flank stimulation in female rats. Lordosis appears to be regulated in part by the synthesis of proteins in the ventral medial hypothalamus (VMH) that are transported to the midbrain central gray (MCG). We developed a strategy involving microinfusion of radioactive amino acids, followed by 2-dimensional gel electrophoresis...

let r be a commutative noetherian ring. we study the behavior of injectiveand at dimension of r-modules under the functors homr(-,-) and -×r-.

We consider general properties of central charges of zero-branes and associated duality invariants, in view of their double role, on the bulk and on the world volume (quantum mechanical) theory. A detailed study of the BPS condition for the mass spectrum arising from toroidal compactifications is given for 1/2, 1/4 and 1/8 BPS states in any dimension. As a byproduct, we retrieve the U-duality i...

We give a new representation of fractional Brownian motion with Hurst parameter H < 1 2 using stochastic partial differential equations. This representation allows us to use the Markov property and time reversal, tools which are not usually available for fractional Brownian motion. We then give simple proofs that fractional Brownian motion does not hit points in the critical dimension, and that...

In this article we study a normalized double obstacle problem with polynomial obstacles p ≤ p under the assumption p(x) = p(x) iff x = 0. In dimension n = 2 we give a complete characterization of blow-up solutions depending on the coefficients of the polynomials p, p. We see that there exists a new type of blow-ups, that we call double-cone solutions since the noncoincidence set is a union of t...