Genome wide transcription analysis in response to stresses is important to provide a basis of effective engineering strategies to improve stress tolerance in crop plants. We assembled a Brassica rapa oligomeric microarray (Br135K microarray) using sequence information from 41,173 unigenes and analyzed the transcription profiles of two contrasting doubled haploid (DH) lines, Chiifu and Kenshin, ...

Stem rust, caused by Puccinia graminis f. sp. tritici, is a destructive disease of wheat that can be controlled by deploying effective stem rust resistance (Sr) genes. Highly virulent races of P. graminis f. sp. tritici in Africa have been detected and characterized. These include race TRTTF and the Ug99 group of races such as TTKSK. Several Canadian and U.S. spring wheat cultivars, including t...

Limited genetic variability is a major problem in rapeseed breeding, whose strict selection, limited geographical range and short period of domestication has led to reduction phenotypic diversity. Assessing specific populations for the greatest diversity many traits simultaneously, requires evaluation multi-feature characteristics. The aim this study was estimate DH (doubled haploid) lines two ...

Factors affecting reliable plant regeneration from unfertilized ovule culture of gentians (Gentiana spp.) were examined. Cold pretreatment (4°C) of flower buds enhanced or maintained production of embryo-like structure (ELS). When 43 genotypes were surveyed in two different labs, 40 of them produced ELSs ranging from 0.01 to 26.5 ELSs per flower bud. No ELSs could be obtained in three genotypes...

Seed germination and seedling establishment are the most critical stages in barley (Hordeum vulgare ssp. L.) life cycle that contribute substantially to grain yield. These two phases exposed several forms of environmental stresses such as salinity due high level salt accumulation soil rhizosphere where seed takes place seedlings emerge from. Previously, we have reported genotypic variability in...

Microspores can be induced to develop homozygous doubled haploid plants in a single generation. In the present experiments androgenic microspores of wheat have been genetically transformed and developed into mature homozygous transgenic plants. Two different transformation techniques were investigated, one employing electroporation and the other co-cultivation with Agrobacterium tumefaciens. Di...

Maize (Zea mays L.) is a plant that grown for human and animal consumption, as well biofuel range of industrial applications throughout the world. By 2020, it predicted to outnumber wheat rice in terms demand. Farmers pick among two types maize varieties modern agriculture. Hybrids open-pollinated are mostly influenced by current environmental economic conditions availability seed preferred var...