Peptides presented by major histocompatibility complex class II (MHCII) molecules to CD4+ T cells play a central role in the initiation of adaptive immunity. This antigen presentation process is characterized by the proteolytic cleavage of foreign and self proteins, and loading of the resultant peptides onto MHCII molecules. Loading and exchange of antigenic peptides is catalyzed by a non-class...

To investigate a conformational change accompanying peptide binding to class II MHC proteins, we probed the structure of a soluble version of the human class II MHC protein HLA-DR1 in empty and peptide-loaded forms. Peptide binding induced a large decrease in the effective radius of the protein as determined by gel filtration, dynamic light scattering, and analytical ultracentrifugation. It cau...

Swi2/Snf2 ATPases remodel substrates such as nucleosomes and transcription complexes to control a wide range of DNA-associated processes, but detailed structural information on the ATP-dependent remodeling reactions is largely absent. The single subunit remodeler Mot1 (modifier of transcription 1) dissociates TATA box-binding protein (TBP):DNA complexes, offering a useful system to address the ...

We have previously mapped a repression domain from the active transcriptional repressor E4BP4 to a 65 amino acid segment near the C-terminus of the polypeptide. Here we show that the E4BP4 repression domain interacts specifically with the TBP binding repressor protein Dr1. Mutants that affect the ability of E4BP4 to bring about transcriptional repression are also deficient in their binding of D...

Abstract We describe the Dark Energy Survey (DES) photometric data set assembled from first three years of science operations to support DES Year 3 cosmologic analyses, and provide usage notes aimed at broad astrophysics community. Y3 GOLD improves on previous releases DES, Y1 , Data Release 1 (DES DR1), presenting an expanded curated that incorporates algorithmic developments in image detrendi...

The molecular structure, UV-visible spectra, and optical properties of D-π-A conjugated organic dye molecules (Disperse Red 1 (DR1) Disperse 73 (DR73)) were analyzed using density functional theory (DFT) time-dependent (TD-DFT) compared with azobenzene molecule to study the effect donor acceptor substituents on properties. performance DFT functionals is investigated B3LYP hybrid three long-rang...

With Gaia’s first data release comes a large amount of new data on stars in the Milky Way. We were interested in a subset of these that were cross referenced with the Tycho-2 catalogue. In this project, we look at two ways of analyzing the data spatial correlation functions and velocity profiles. To find a velocity profile, we looked at a subset of stars along the sun to galactic center line to...

MHC class II molecules (MHC II) play a pivotal role in the cell-surface presentation of antigens for surveillance by T cells. Antigen loading takes place inside the cell in endosomal compartments and loss of the peptide ligand rapidly leads to the formation of a non-receptive state of the MHC molecule. Non-receptiveness hinders the efficient loading of new antigens onto the empty MHC II. Howeve...