Retinal damage is an optional sign in a number of neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS). The aim this work was to assess the structural and functional state retina murine model ALS caused by overexpression aberrant FUS protein [1-359]. retinal examination carried out on 12 transgenic 13 wild-type mice 2.5–3 months age. study revealed not statistically signifi...

A life-threatening manifestation of Covid-19 infection is a cytokine storm that requires hospitalization and supplemental oxygen. Various strategies to reduce inflammatory cytokines have had some success in limiting improving survival. Agonists adenosine A2A receptors (A2AR) release from most immune cells. Apadenoson potent selective anti-inflammatory analog reduces inflammation. When administe...

Adalimumab is the only FDA- and EMA-approved treatment for moderate to severe hidradenitis suppurativa (HS), where etanercept certolizumab-pegol have been shown be inefficacious, suggesting that mechanism of action (MOA) adalimumab distinct in HS may contribute improved wound healing. We demonstrated adalimumab, but neither nor certolizumab-pegol, induces a healing profile vitro by regulating m...

Angiotensin-converting enzyme 2 (ACE2) degrades angiotensin II to angiotensin-(1-7) and is expressed in podocytes. Here we overexpressed ACE2 in podocytes in experimental diabetic nephropathy using transgenic methods where a nephrin promoter drove the expression of human ACE2. Glomeruli from these mice had significantly increased mRNA, protein, and activity of ACE2 compared to wild-type mice. M...

BACKGROUND Hepatitis C virus (HCV) is a major cause of chronic hepatitis and a health problem affecting over 170 million people around the world. We previously studied transgenic mice that express HCV Core, Envelope 1 and Envelope 2 proteins predominantly in the liver, resulting in steatosis, liver and lymphoid tumors, and hepatocellular carcinoma. Herein, the immune-mediated cell response to h...

Lymphedema (tissue swelling caused by an accumulation of lymph fluid) in the lower legs is relatively common elderly people. Dermal fibrosis and hyperkeratotic verrucous lesions setting long-standing lymphedema, called elephantiasis verrucosa nostra, can develop some patients, although mechanisms underlying these tissue changes are unknown. Here, we studied effects lymphedema on skin using k-cy...

To determine whether increased levels of VEGF disrupt postnatal lung formation or function, conditional transgenic mice in which VEGF 164 expression was enhanced in respiratory epithelial cells were produced. VEGF expression was induced in the lungs of VEGF transgenic pups with doxycycline from postnatal day 1 through 2 and 6 wk of age. VEGF levels were higher in bronchoalveolar lavage fluid (B...

Materials and Methods Transgenic mice. Transgenic mice homozygous for a human PrP 129V transgene array and murine PrP null alleles (Prnp) designated Tg(HuPrP129V Prnp)-152 mice (129VV Tg152 mice) and transgenic mice homozygous for a human PrP 129M transgene array and murine PrP null alleles (Prnp) designated Tg(HuPrP129M Prnp)-35 mice (129MM Tg35 mice) have been described previously (S1-S4). In...

Type 2 diabetes is characterized by impaired -cell function, hyperglycemia, and islet amyloid deposition. The primary constituent of islet amyloid is the 37–amino acid -cell product called islet amyloid polypeptide (IAPP) or amylin. To study mechanisms of islet amyloid formation, we developed a transgenic mouse model that produces and secretes the amyloidogenic human IAPP (hIAPP) molecule and h...

Maternally inherited familial hypercholesterolemia (FH) impairs glucose metabolism and increases cardiovascular risks in the offspring to a greater degree than paternal inherited FH. However, it remains unknown whether hypertriglyceridemia affects glucose metabolism via inheritance. In this study, we sought to compare the impact of maternally and paternally inherited hypertriglyceridemia on glu...