In Drosophila melanogaster there are two genes which encode the enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH), Gapdh-43E and Gapdh-13F. We have shown that Gapdh-43E codes for the GAPDH subunit with an apparently larger molecular weight while Gapdh-13F encodes the GAPDH subunit having an apparently smaller molecular weight. Immunoblots of sodium dodecyl sulfate gels were used to survey...

Fruit-flies of the genus Drosophila are characterized by overwhelming variation in fertilization traits such as copulatory plug formation, sperm storage organ use, and nutritional ejaculatory donation. Despite extensive research on the genetic model Drosophila melanogaster, little is known about the molecular underpinnings of these interspecific differences. This study employs a proteomic appro...

Although many benefits drawn from beer consumption are claimed, the epidemiological records contradictory with respect to cancer prevention. The purpose of this study was investigate possible health-related activities involving genome safety and ageing processes two types lyophilised ale beers (blond stout), as well their bioactive compounds (tyrosol iso-alpha humulone). A multipurpose trial se...

BACKGROUND Systematic, large-scale RNA interference (RNAi) approaches are very valuable to systematically investigate biological processes in cell culture or in tissues of organisms such as Drosophila. A notorious pitfall of all RNAi technologies are potential false positives caused by unspecific knock-down of genes other than the intended target gene. The ultimate proof for RNAi specificity is...

Using PCR and sequencing we have demonstrated the presence of P elements in genomes of Drosophila melanogaster from natural populations of Ukraine. The degree of gonadal reduction revealed indirectly indicates low or, in some cases, zero activity of the mobile element in the inspected natural populations of Drosophila. All studied populations have been found to represent the M’ cytotype. In thi...

This article reports the breaking of ethological barriers through the constitution of soma-germ line chimeras between species of the melanogaster subgroup of Drosophila, which are ethologically isolated. Female Drosophila yakuba and D. teissieri germ cells in a D. melanogaster ovary produced functional oocytes that, when fertilized by D. melanogaster sperm, gave rise to sterile yakuba-melanogas...

Abstract Classical biological control, i.e., the introduction of natural enemies from an invasive pest’s area origin, has been proposed repeatedly to control spotted wing drosophila Drosophila suzukii in Americas and Europe. Results surveys Asia laboratory experiments suggest parasitoid G1 Ganaspis cf. brasiliensis as a suitable agent. To study host specificity under semi-field conditions, we c...

Background: In this study, the toxicity of the different xenobiotics was tested on the fruit fly Drosophila melanogaster model system.  Methods: Fly larvae were raised on food supplemented with xenobioticsat different concentrations (sodium nitroprusside (0.1-1.5 mM), S-nitrosoglutathione (0.5-4 mM), and potassium ferrocyanide (1 mM)). Emergence of flies, food intake by larvae, and pupation h...

The process of aging is a complex phenomenon that involves progressive decline in physiological functions required for survival and fertility. To better understand the mechanisms underlying this process, scientific community has utilized several tools. Among them, mitochondrial DNA emerged as crucial factor biological aging, given dysfunction thought to significantly contribute phenomenon. Addi...