BACKGROUND In higher plants, inorganic nitrogen is assimilated via the glutamate synthase cycle or GS-GOGAT pathway. GOGAT enzyme occurs in two distinct forms that use NADH (NADH-GOGAT) or Fd (Fd-GOGAT) as electron carriers. The goal of the present study was to characterize wheat Fd-GOGAT genes and to assess the linkage with grain protein content (GPC), an important quantitative trait controlle...

The observations of a long-term experiment on the use of saline water were used to compare the crop tolerance to salinity. Salinity affected significantly yield, evapotranspiration, pre-dawn leaf water potential and stomatal conductance. The higher the salinity, the lower the yield, evapotranspiration, pre-dawn leaf water potential and stomatal resistance. The crop classification, based on soil...

A major problem for durum wheat production in the Mediterranean region is yield fluctuation. This fluctuation is a result of year-to-year variation in precipitation and heat stress during grain growth, which is typical of the Mediterranean climate. Both yield stability and good quality are needed in adapted durum wheat ideotypes. Ten durum wheat cultivars differing in drought resistance were gr...

The action of species cytoplasm specific (scs) gene(s) can be observed when a durum (Triticum turgidum L.) nucleus is placed in the Aegilops longissimum S. & M. cytoplasm. This alloplasmic combination, (lo) durum, results in nonviable progeny. A scs gene derived from T. timopheevii Zhuk. (scs(ti)) produced compatibility with the (lo) cytoplasm. The resulting hemizygous (lo) scs(ti)- durum line ...

Production of doubled haploids (DHs) is an important methodology to speed the process of breeding and development of mapping populations in crops. The procedure for DH production includes two major steps: haploid induction and chromosome doubling. In recent years, wide hybridization between wheat and maize has become a main approach for haploid production in wheat. In this method, the maize chr...

The development of a robust Agrobacterium-mediated transformation protocol for a recalcitrant species like durum wheat requires the identification and optimization of factors affecting T-DNA delivery and plant regeneration. The purpose of this research was to compare the behavior of diverse durum wheat genotypes during in vitro culture and Agrobacterium tumefaciens-mediated transformation, usin...

The main objective of this study was to examine the effects of NaCl on the leaf growth and some physiological and biochemical characteristics of an Algerian durum wheat (var. Waha) landrace. NaCl (150 mM), as compared to control plants, significantly reduced all the leaf-growth parameters as leaf area (33%), leaf dry weight (41%) and leaf fresh weight (32%), but did not induce any change in the...

Cytological and agronomic characteristics of a F2 population from Triticum aestivum L. x T. durum Desf. hybrids were analyzed plant by plant. Means of morphologic traits in the F2 population were similar to those of the low-value parent. On average, F2 hybrids had 36.54 chromosomes per plant, indicating that each gamete lost 2.73 chromosomes at meiosis of the F1 generation. More than half of pl...

The reduced risk of chronic diseases related to whole grain consumption is in part attributed to their high antioxidant content. Many studies have been performed on the in vitro antioxidant capacity of cereals, but in vivo studies are necessary. We have evaluated and compared the effect of whole grain durum wheat bread and whole grain Kamut khorasan bread on the oxidative status in rats. Two di...

Stability of grain quality characteristics is an interesting feature of today’s durum wheat breeding programmes, due to the high annual variation in both grain yield and quality, particularly in the Mediterranean area. Ten field trials were carried out during two seasons (1998 and 1999) in both the north (Lleida) and south (Granada and Jerez) of Spain. Ten durum wheat genotypes were used, inclu...