Teh ultrasound-assisted magnetic stirrer was used as an effective extraction technique for teh evaluation of total phenolic contents (TPC) and antioxidant potential of ten Pakistani brown rice cultivars. For this purpose, ultrasonic (240W frequency 50/60 Hertz) assisted magnetic stirrer coupled with three solvents methanol, ethanol, and isopropanol in pure and aqueous fractions (80:20) were...

There is an increasing requirement for assessment of the bioavailable metal fraction and the mobility of trace elements in soils upon disposal. One of the approaches is the use of leaching procedures, but the results obtained are operationally defined; therefore, their significance is highly dependent on the extraction protocol performed. So, for this type of study, there is a need for referenc...

Plastocyanin is released from chloroplasts by sonication, by passing through the French-press, by extraction with detergents, by treatment with organic solvents and by freezing and thawing. No plastocyanin is liberated during osmotic shock of either class I or class II chloroplasts, or by extraction of class II chloroplasts with dilute solutions of EDTA. Under special conditions it is possible ...

The mechanism of DNA degradation and its clinical applications were examined. When purified lambda phage and extracted liver DNA were fixed in phosphate buffered formaldehyde, the DNA did not degrade, but there was incomplete digestion with endonuclease. Rat liver tissues were fixed under various conditions and DNA extracted. Immediate fixation with buffered formaldehyde at low temperature, or ...

Termine et al. first demonstrated that sequential dissociative extraction and fractionation procedures with protease inhibitors could provide a convenient approach for the study of mineral compartment constituents. The primary extraction regimen used 4 M guanidine HCl to remove most of the protein from the nonmineralized phase of bone. Subsequently, EDTA-guanidine was used to remove the mineral...

A modest liquid chromatography/tandem mass spectrometric method was developed and validated to determine three tetracycline antibiotics and their epimers tetracycline, oxytetracycline, chlortetracycline, 4-epi tetracycline, 4-epi oxytetracycline, 4-epi chlortetracycline in shrimp samples respectively. The procedure was involved the extraction of tetracyclines and its epimers from homogenized sa...

Several DNA extraction methods have been used for formalin-fixed, paraffin-embedded tissues, with variable results being reported regarding the suitability of DNA obtained from such sources to serve as template in polymerase chain reaction (PCR)-based genetic analyses. We present a method routinely used for archival material in our laboratory that reliably yields DNA of sufficient quality for P...

A multiresidue quantitative screening method covering 39 antibiotics from 7 different families by ultra-high-pressure-liquid-chromatography-tandem mass spectrometry (UHPLC-MS/MS) is described. Sulfonamides, trimethoprim, tetracyclines, macrolides, quinolones, penicillins and chloramphenicol are simultaneously detected in liver tissue. A simple sample treatment method consisting of extraction wi...