Neurotoxic shellfish poisoning is a significant food-borne disease caused by potent cyclic polyether toxins (brevetoxins) that accumulate in the flesh of shellfish. Here we report a new procedure for brevetoxin analysis by capillary electrophoresis-based immunoassay (CE-IA) with electrochemical (EC) detection. In this method, after the competitive immunoreaction in liquid phase, the horseradish...

Prostate specific antigen (PSA) is a prominent marker for the prostate carcinoma. It is found in human blood in free (f-PSA) and complex forms. These two forms together are called total PSA (t-PSA). Estimation of both forms is essential to predict malignancy. In this study we report a unique and effective technique of electrochemical detection of fPSA using magnetic beads on a three-electrode s...

The deflection of cantilever systems may be performed by an indirectelectrochemical method that consists of measuring the local cantilever activity anddeflection in a feedback generation-collection configuration of the SECM. This isillustrated during the electrochemically-assisted adsorption of Br onto a gold coatedcantilever, either in its pristine state or previously coated wi...

A simple amino-functionalization method for carbon nanotubes and its application in an electrochemical immunosensor for detection of the human cardiac troponin T are described. Amino-functionalized carbon nanotubes allow oriented antibodies immobilization via their Fc regions, improving the performance of an immunosensor. Herein multiwalled carbon nanotubes were amino-functionalized by using th...

This work proposed a simple, sensitive and low-cost multiplexed immunoassay by combining a disposable chip with gold nanoparticle (AuNP) as an electrochemical label. The immunosensors array as the disposable chip was firstly prepared by immobilizing capture antibodies on different screen printed carbon working electrodes by passive adsorption. With a sandwich mode, the analytes were then bound ...

Analysts are always interested in finding new functional nanomaterials and devices with good properties for electrochemical sensor applications. In this paper, hyperbranched polyester nanoparticles with carboxylic acid functional groups (HBPE-CA NPs) were synthesized and combined with chitosan wrapped around Au nanoparticles (CS-Au NPs) to prepare a novel and sensitive electrochemical immunosen...

− This paper introduces a novel electrochemical detection method for measuring the formation of an immunological complex. The described electrochemical immunoassay consists of several consecutive steps. First the sample is mixed with silver colloid and magnetic particles both previously conjugated to appropriate antibodies. The analyte from the sample binds to both silver and magnetic beads for...

A novel nonseparation electrochemical enzyme immunoassay (NEEIA) for detecting marker proteins in undiluted blood is described. The approach is based on preferential electrochemical measurement of surface-bound enzyme-labeled reporter antibody (E-Ab), relative to an excess of this reagent in the sample solution. NEEIAs are carried out on microporous membranes coated with a thin, circular area o...

A novel electrochemical immunosensor array for the simultaneous detection of multiple tumor markers was developed by incorporating electrochemically addressing immobilization and one signal antibody strategy. As a proof-of-principle, an eight-electrode array including six carbon screen-printed working electrodes was used as a base array for the analysis of two important tumor markers, carcinoem...

Bacterial food poisoning is an ever-present threat that can be prevented with proper care and handling of food products. A disposable electrochemical immunosensor for the simultaneous measurements of common food pathogenic bacteria namely Escherichia coli O157:H7 (E. coli), campylobacter and salmonella were developed. The immunosensor was fabricated by immobilizing the mixture of anti-E. coli, ...