By using a recombinant severe acute respiratory syndrome coronavirus (SARS-CoV) nucleocapsid protein-based enzyme-linked immunosorbent assay (ELISA) and serum specimens serially collected (from day 0 to day 240 after symptom onset) from patients with pneumonia due to SARS-CoV, we analyzed the longitudinal profiles of immunoglobulin G (IgG), IgM, and IgA antibodies against the SARS-CoV nucleocap...

The precise diagnosis of the acute toxoplasmosis in pregnant women and immunocompromsied patients has critical importance. Most of the commercially available assays use the whole Toxoplasma soluble extract as the antigen. However, the assays currently available for the detection of specific anti-Toxoplasma antibodies may vary in their abilities to detect serum immunoglobulins, due to the lack o...

INTRODUCTION Typhoid fever is endemic in many parts of the world and represents a major cause of acute febrile illness (AFI). Rapid and accurate laboratory methods for diagnosis of this disease are needed for both patient care and surveillance situations. METHODOLOGY Serum samples were collected from AFI patients and used to evaluate the performance of a newly developed ELISA assay that uses ...

BACKGROUND AND OBJECTIVES Timely diagnosis of leptospirosis is essential for an effective treatment. Large diversity of clinical symptoms has led leptospirosis diagnosis difficult. Researchers have conducted many tests with wide-range of sensitivity and specificity to achieve novel diagnostic procedures which have higher sensitivity and specificity compared with previous tests and which are mor...

Introducción: Las pruebas inmunológicas son utilizadas como diagnóstico complementario en la vigilancia epidemiológica. Objetivos: Evaluar prueba inmunocromatográfica para detección de anticuerpos IgM e IgG contra el SARS-CoV-2, usando referencia ELISA IgM/IgG COVID-19. Metodología: El estudio realizado comparó inmunocromatográficas con IgM/IgG. También se efectuó estimación sensibilidad y espe...

BACKGROUND This study is performed to reveal most common species and subspecies of leptospires that are main causes of human leptospirosis in Guilan, Northern Province of Iran. METHODS We performed IgM-ELISA and MAT on 282 blood samples from patients who attended to 3 hospitals in the flat area of Guilan Province with clinical symptoms consisted with leptospirosis. All specimens with titers ≥...

In this study, we examined the diagnostic accuracy of the InBios Scrub Typhus Detect IgM enzyme-linked immunosorbent assay (ELISA) and determined the optimal diagnostic optical density (OD) cutoffs for screening and diagnostic applications based on prospectively collected, characterized samples from undifferentiated febrile illness patients in northern Thailand. Direct comparisons with the sero...

We read with interest the study by Diederen et al. (1), in which the serological assays from Vircell were evaluated. We found that this work underlines the importance of immunoglobulin M (IgM) detection in the diagnosis of legionellosis previously described by our group (3). Yet, we would like to point out some disagreements with some of the information given in the aforementioned study. The au...

BACKGROUND Toxoplasmosis is a serious disease in immunocompromised patients and pregnant women. Differentiation of acute and chronic infection is a major challenge in serodiagnosis of the disease. Since the aim of this study was to assess the diagnostic utility of recombinant SAG1 (rec-SAG1) for the detection of Toxoplasma-specific IgM antibodies in human sera, by an enzyme-linked immunosorbent...

BACKGROUND & OBJECTIVES Scrub typhus is a vector-borne zoonotic infection caused by Orientiatsutsugamushi. Local epidemiology of the circulating serotypes of scrub typhus is not available from most parts of India. We conducted this study for the diagnosis of scrub typhus using IgM ELISA and to detect O. tsutsugamushi serotypes circulating in southern Andhra Pradesh, India. METHODS Samples wer...