We report a case of right adnexal torsion during pregnancy after an oocyte in vitro maturation and intracitoplasmic sperm injection cycle in patient with polycystic ovary syndrome. A 31-year-old woman with a typical clinical disorder of polycystic ovarian syndrome was included in an oocyte in vitro maturation program. Right adnexal torsion occurred two days after embryo transfer, and laparoscop...

Oocytes matured in vitro are useful for assisted human and farm animal reproduction. However, the quality of oocytes is usually lower than that vivo oocytes, possibly due to absence some important signal regulators vitro. In this study, untargeted metabolomics was used detect changes metabolites follicular fluid (FF) during pig oocyte maturation culture medium maturation. Our results showed tot...

Previous cases reported recurrent all oocytes in repeated cycles were arrested at MI failed to mature after culture and ICSI resulted total nonfertilization. Ionomycin induced first PB extrusion patients with maturation-resistant oocytes. combined assisted oocyte activation (AOA) on MII matured vitro abnormal fertilization no further embryo cleavage potential. The objective of this work was res...

Although the mechanisms under which sex ratio is controlled in nature remain unknown, researchers have attempted to treat sperm in an effort to predetermine the sex of the offspring. The methods of sperm separation that have been repeatedly reported to isolate fractions rich in X- or Y-chromosome bearing sperm are serum albumin gradient swim-up and flow cytometry. However, the ability of the se...

The present study aims to determine the potential of Bali cattle ovaries as sources oocytes for in vitro embryo production based on different ovarian reproductive statuses. were grouped into 4 categories: with no corpus luteum and dominant follicles (CL-DF-), those (CL+DF-), without but (CL-DF+), follicle (CL+DF+). collected via slicing technique grades (a, b, c, d). oocyte’s maturation was per...

Developing a culture system for preantral follicles has important biotechnological implications due to the potential to produce a large number of oocytes for embryo production and transfer. To accomplish this goal, the present study was aimed to culture preantral follicles in the presence of different media, sera and FSH concentrations. Six-week-old preantral follicles (95 ± 5 µm) were cultured...

Prevalence of Polycystic Ovarian Syndrome(PCOS) in Iran is more than 6%. Therefore we encounter with many PCOS patients. In Vitro Maturation (IVM) of oocytes as an attractive method in ART is considered. Studies show that changes in culture conditions should be administered to make IVM protocol more successful .For this purpose in this study we have set up the beneficial cultures for IVM proced...

The aetiology of azoospermia can be grossly divided into obstructive and non-obstructive causes. Although in both cases testicular spermatozoa can be used to treat male fertility, it is not well established whether success rates following intracytoplasmic sperm injection (ICSI) are comparable. Therefore, a retrospective analysis of fertilization, pregnancy and embryo implantation rates was perf...