Background Anaphylaxis after vaccination is rare but significant problem since it may be fatal without prompt treatment. Proper diagnosis and identification of the causative agent is critical for management and prophylaxis of the anaphylaxis. Influenza vaccine-associated anaphylaxis (IVA) has been related to egg allergy since influenza vaccines are produced in embryonated eggs. However, patient...

Newcastle disease virus (NDV) was isolated and identified for the first time from a flock of Japanese quail suspected to ND in Iran. Birds of the flock aged variously, and some of them died from the disease. Loss of appetite, weakness and decrease in egg production, diarrhea and nervous symptoms were the main clinical signs in the birds. Hemorrhagic lesions of the intestinal tracts and proventr...

Salmonella paratyphi B and Salmonella typhimurium were starved in seawater microcosms respectively for 12 and 6 years. After starvation periods, major part of water was evaporated in each microcosm and salt crystals appeared. No culturable cells were obtained in selective and non-selective media. Salty suspension of each microcosm was inoculated in the yolk sacs of embryonated chicken eggs. Cul...

Chicken Egg Yolk antibodies (IgY) were raised in 24 week old white leg horn chickens against Streptococcus mitis (MTCC 2696). The chickens received Booster injections of increasing concentrations of antigen to raise the antibody level in egg yolk. The antibodies were purified from immunized chicken egg yolk by Poly ethylene Glycol (PEG) and Ammonium sulphate precipitation method and further pur...

A recombinant filamentous phage bearing the peptide sequence CTLTTKLYC has been shown to interact tightly with Newcastle disease virus (NDV). The peptide, either alone or in the phage associated form, inhibited the hemolytic activity of the virus and also its propagation in embryonated chicken eggs. Here, we demonstrate that phage bearing the peptide sequence CTLTTKLYC and a synthetic peptide d...

Positron emission tomography/computer tomography (PET/CT) is an established method in preclinical research in small animal disease models and the clinical diagnosis of cancer. It combines functional information of the positron-emitting biomarker with the anatomical data obtained from the CT image. Thus, it allows for 4D in vivo investigation of biological processes. Recently, PET/CT was used to...

Crystalline turkey egg white lysozyme has been prepared by a simple procedure consisting of adsorption on carboxymethylcellulose followed by elution with (NH4)&03 solution and crystallization from 5 % NaCl solution. The twice crystallized enzyme gave a single peak when eluted from carboxymethylcellulose, and it migrated as a single band during disc electrophoresis. Separation of the tryptic pep...

These results show that primary chick embryo cells in culture release a particle bound RNA dependent DNA polymerase into the culture fluid. The enzyme is also found in the allantoic fluid of embryonated chicken eggs. This phenomenon is not restricted to the chicken system because primary quail embryo cells which are not known to express the so far known endogenous viruses release a particle bou...

To detect the presence of infectious bronchitis virus (IBV) in infected allantoic fluid (AF) of SPF embryonated eggs rapid hemagglutination (HA) activity after treatment with neuraminidase enzyme was used. Twenty IBV suspected materials were inoculated in SPF embryonated eggs via chorioallantoic cavity. Harvested AFs were treated with neuraminidase enzyme and the presence of IBV was detected by...