For the purpose of studying the aging changes of macromolecular synthesis in the colonic cells of experimental animals, we studied 10 groups of aging mice during aging from fetal day 19 to postnatal month 24. They were injected with 3H-leucine, a precursor for protein synthesis, sacrificed and the colonic tissues were processed for light and electron microscopic radioautography. On many radioau...

Tritiated thymidine was administered to four groups of embryos at various stages of development. The first group was killed 45 min after isotope administration. The second group received the isotope three times over a 12 h period, the third group four times over a 24 h period, and the last group eight times over a 48 h period. Sagittal histological sections of the embryos were prepared for radi...

To establish a developmental profile of fetal mouse cardiovascular parameters, we analyzed a large body of ultrasound measurements obtained by in utero echocardiography of C57BL/6J fetal mice from embryonic day 12.5 to 19.5 (term). Measurements were obtained using two-dimensional (2D), spectral Doppler and M-mode imaging with standard clinical cardiac ultrasound imaging planes. As these studies...

BACKGROUND The increasing number of transgenic and targeted mutant mice with embryonic cardiac defects has resulted in the need for noninvasive techniques to examine cardiac structure and function in early mouse embryos. We report the first use of a novel 40-MHz ultrasound imaging system in the study of mouse cardiac development in utero. METHODS AND RESULTS Transabdominal scans of mouse embr...

From Greek antiquity to the era of computer science, astronomical refraction has raised questions about real angular position celestial bodies as seen from Earth surface, structure its atmosphere, and navigation. After introducing embryonic ideas first measurements, article presents early founders a mathematically structured study: Cassini, Newton, Taylor—the last two inventing appropriate math...

Before septation processes shape its four chambers, the embryonic heart is a straight tube that spontaneously bends and twists breaking left-right symmetry. In particular, sub...

The reproductive cycle of the Australian sharpnose shark, Rhizoprionodon taylori, includes a temporary suspension of development at the commencement of embryogenesis termed embryonic diapause. This study investigated levels of 17β-estradiol (E2), testosterone (T) and progesterone (P4) in plasma samples of mature wild female R. taylori captured throughout the reproductive cycle and correlated th...

First trimester human embryonic eye globes were micro-dissected so that a passage was opened between the outer environment and the anterior chamber, which rendered free access of tissue culture medium to the endothelial cell monolayer. The dissected eye globes were maintained in organ culture for 24h in the continuous presence of tritiated thymidine. Sections were cut through the whole eye glob...

Abstract It is estimated that 17.3% of all pregnancies in the horse are lost from time detection to day 40, which has significant economic impact. But still, equine reproductive research limited funding opportunities. Understanding dynamics between embryo, endometrium, and corpus luteum (CL) essential ameliorate those losses. Pig embryonic interferon (IFN) gamma for establishment pregnancy. In ...

Smooth-muscle-cell (SMC) replication and extracellular matrix protein expression are two vital and interrelated processes necessary for normal development of the vasculature. To understand better the nature of this relationship in the developing rat lung, we investigated the relationship between SMC proliferation and the expression of perlecan, a basement membrane (BM) heparan sulfate proteogly...