Increased oxidative stress is an unavoidable consequence of exposure to the space environment. Our previous studies showed that mice exposed to space for 13.5 days had decreased glutathione levels, suggesting impairments in oxidative defense. Here we performed unbiased, unsupervised and integrated multi-'omic analyses of metabolomic and transcriptomic datasets from mice flown aboard the Space S...

We present herein a novel method of pIII-based antibody phage display using Hpd3cells--bacterial cells bearing the genome of a gene-III-lacking helper phage (VCSM13d3). A high level of single-chain variable fragments (scFvs) was displayed in the consequent phagemid particles using Hpd3cells to rescue the phagemid encoding scFv-pIII. Hpd3cells considerably improved the specific enrichment factor...

The Motif Enrichment Tool (MET) provides an online interface that enables users to find major transcriptional regulators of their gene sets of interest. MET searches the appropriate regulatory region around each gene and identifies which transcription factor DNA-binding specificities (motifs) are statistically overrepresented. Motif enrichment analysis is currently available for many metazoan s...

A method has been developed for determining of heavy metal ions by field-amplified sample injection capillary electrophoresis with contactless conductivity detection. The effects of the 2-N-morpholinoethanesulfonic acid/histidine (MES/His) concentration in the sample matrix, the injection time and organic additives on the enrichment factor were studied. The results showed that MES/His with a lo...

in this study multivariate statistical analysis are used to characterize relationships between hydrochemical properties ofthermal and non-thermal springs. four factors for thermal waters and two for non-thermal springs were extracted basedon factor analysis. in thermal springs, the first factor showed high loading on ca, mg, na and k and this factor wasinterpreted as leaching of cations in the ...

In this study multivariate statistical analysis are used to characterize relationships between hydrochemical properties ofthermal and non-thermal springs. Four factors for thermal waters and two for non-thermal springs were extracted basedon factor analysis. In thermal springs, the first factor showed high loading on Ca, Mg, Na and K and this factor wasinterpreted as leaching of cations in the ...

Reduced bioavailability of nonpolar contaminants due to sorption to natural organic matter is an important factor controlling biodegradation of pollutants in the environment. We established enrichment cultures in which solid organic phases were used to reduce phenanthrene bioavailability to different degrees (R. J. Grosser, M. Friedrich, D. M. Ward, and W. P. Inskeep, Appl. Environ. Microbiol. ...

Environmental enrichment produces protective addiction and depression phenotypes in rats. ΔFosB is a transcription factor that regulates reward in the brain and is induced by psychological stress as well as drugs of abuse. However, the role played by ΔFosB in the protective phenotypes of environmental enrichment has not been well studied. Here, we demonstrate that ΔFosB is differentially regula...

Harsh environments are typically characterized by some dominant variable limiting diversity, making them interesting systems for studying how species diversity patterns change with abiotic conditions. Several environmental factors with the potential to influence phytoplankton diversity in hypersaline lakes were examined with microcosm experiments using inoculum from the Great Salt Lake, Utah. E...

The Loess Plateau is a fragile ecological zone that sensitive to climate change. response, adaptation, and feedback of tree growth in forest ecosystems global warming CO2 enrichment are urgent scientific issues. Intrinsic water use efficiency (iWUE) an important indicator for understanding ecosystem adaptability change enrichment. In this study, tree-ring width, stable carbon isotope ratio (?13...